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Pasteurization of bone for tumour eradication prior to reimplantation – An in vitro & pre-clinical efficacy study

BACKGROUND & OBJECTIVES: In current era of limb-salvage therapy, pasteurization of bone sarcomas is receiving growing attention as a potential extracorporeal treatment and cost-effective alternative to allografts and radiation before surgical reimplantation. Detailed in vitro and in vivo pre-cli...

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Autores principales: Kode, Jyoti, Taur, Prasad, Gulia, Ashish, Jambhekar, Nirmala, Agarwal, Manish, Puri, Ajay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078498/
https://www.ncbi.nlm.nih.gov/pubmed/24927346
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author Kode, Jyoti
Taur, Prasad
Gulia, Ashish
Jambhekar, Nirmala
Agarwal, Manish
Puri, Ajay
author_facet Kode, Jyoti
Taur, Prasad
Gulia, Ashish
Jambhekar, Nirmala
Agarwal, Manish
Puri, Ajay
author_sort Kode, Jyoti
collection PubMed
description BACKGROUND & OBJECTIVES: In current era of limb-salvage therapy, pasteurization of bone sarcomas is receiving growing attention as a potential extracorporeal treatment and cost-effective alternative to allografts and radiation before surgical reimplantation. Detailed in vitro and in vivo pre-clinical study to evaluate efficacy of pasteurization to eradicate malignant cells has not been reported yet. The present study was carried out to assess the efficacy of pasteurization to kill tumour cells both in vitro and in vivo. METHODS: Surgically resected specimens of osteosarcomas (n=4) were cut into equal halves and one section was pasteurized by heating at 60°C to 65°C for 40 min. Paired samples before and after pasteurization were studied in vitro for DNA ploidy, evaluation of histological change and elimination of mitotic activity. These tissues were transplanted in immune-deficient NOD-SCID mice to evaluate effect on tumour-generating ability, presence of human nuclei, osteopontin and cytokine/chemokines released in tumour-transplanted mice. RESULTS: Non-pasteurized tumour samples had viable tumour cells which exhibited significant growth in culture, increased proliferative ability and clonogenic potential while respective pasteurized tumour tissues did not grow in culture and did not exhibit clonogenicity. Flow cytometry revealed that propidium iodide positive dead cells increased significantly (P< 0.01) post pasteurization. Seven of 12 non-pasteurized tumour transplanted mice demonstrated tumour-forming ability as against 0 of 12 in pasteurized tumour transplanted mice. Solid tumour xenografts exhibited strong expression of anti-human nuclei and osteopontin by immunohistochemistry as well as secretary human interluekin-6 (IL-6) while pasteurized mice failed to express these markers. INTERPRETATION & CONCLUSIONS: This study has provided a basis to establish pasteurization as being efficacious in ensuring tumour eradication from resected bone tumour specimens. Pasteurized tumour bearing bone can thus safely be used to reconstruct large defects after tumour resection.
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spelling pubmed-40784982014-07-02 Pasteurization of bone for tumour eradication prior to reimplantation – An in vitro & pre-clinical efficacy study Kode, Jyoti Taur, Prasad Gulia, Ashish Jambhekar, Nirmala Agarwal, Manish Puri, Ajay Indian J Med Res Original Article BACKGROUND & OBJECTIVES: In current era of limb-salvage therapy, pasteurization of bone sarcomas is receiving growing attention as a potential extracorporeal treatment and cost-effective alternative to allografts and radiation before surgical reimplantation. Detailed in vitro and in vivo pre-clinical study to evaluate efficacy of pasteurization to eradicate malignant cells has not been reported yet. The present study was carried out to assess the efficacy of pasteurization to kill tumour cells both in vitro and in vivo. METHODS: Surgically resected specimens of osteosarcomas (n=4) were cut into equal halves and one section was pasteurized by heating at 60°C to 65°C for 40 min. Paired samples before and after pasteurization were studied in vitro for DNA ploidy, evaluation of histological change and elimination of mitotic activity. These tissues were transplanted in immune-deficient NOD-SCID mice to evaluate effect on tumour-generating ability, presence of human nuclei, osteopontin and cytokine/chemokines released in tumour-transplanted mice. RESULTS: Non-pasteurized tumour samples had viable tumour cells which exhibited significant growth in culture, increased proliferative ability and clonogenic potential while respective pasteurized tumour tissues did not grow in culture and did not exhibit clonogenicity. Flow cytometry revealed that propidium iodide positive dead cells increased significantly (P< 0.01) post pasteurization. Seven of 12 non-pasteurized tumour transplanted mice demonstrated tumour-forming ability as against 0 of 12 in pasteurized tumour transplanted mice. Solid tumour xenografts exhibited strong expression of anti-human nuclei and osteopontin by immunohistochemistry as well as secretary human interluekin-6 (IL-6) while pasteurized mice failed to express these markers. INTERPRETATION & CONCLUSIONS: This study has provided a basis to establish pasteurization as being efficacious in ensuring tumour eradication from resected bone tumour specimens. Pasteurized tumour bearing bone can thus safely be used to reconstruct large defects after tumour resection. Medknow Publications & Media Pvt Ltd 2014-04 /pmc/articles/PMC4078498/ /pubmed/24927346 Text en Copyright: © Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kode, Jyoti
Taur, Prasad
Gulia, Ashish
Jambhekar, Nirmala
Agarwal, Manish
Puri, Ajay
Pasteurization of bone for tumour eradication prior to reimplantation – An in vitro & pre-clinical efficacy study
title Pasteurization of bone for tumour eradication prior to reimplantation – An in vitro & pre-clinical efficacy study
title_full Pasteurization of bone for tumour eradication prior to reimplantation – An in vitro & pre-clinical efficacy study
title_fullStr Pasteurization of bone for tumour eradication prior to reimplantation – An in vitro & pre-clinical efficacy study
title_full_unstemmed Pasteurization of bone for tumour eradication prior to reimplantation – An in vitro & pre-clinical efficacy study
title_short Pasteurization of bone for tumour eradication prior to reimplantation – An in vitro & pre-clinical efficacy study
title_sort pasteurization of bone for tumour eradication prior to reimplantation – an in vitro & pre-clinical efficacy study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078498/
https://www.ncbi.nlm.nih.gov/pubmed/24927346
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