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KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE)

BACKGROUND: CASTLE (Carcinoma showing thymus-like elements) is a rare malignant neoplasm of the thyroid resembling lymphoepithelioma-like and squamous cell carcinoma of the thymus with different biological behaviour and a better prognosis than anaplastic carcinoma of the thyroid. METHODS: We retrosp...

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Autores principales: Veits, Lothar, Schupfner, Rupert, Hufnagel, Petra, Penzel, Roland, Freitag, Jens, Ströbel, Philipp, Kern, Michael A, Schröder, Sören, Neuhold, Nikolaus, Schmid, Kurt W, Schirmacher, Peter, Hartmann, Arndt, Rieker, Ralf J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078982/
https://www.ncbi.nlm.nih.gov/pubmed/24934485
http://dx.doi.org/10.1186/1746-1596-9-116
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author Veits, Lothar
Schupfner, Rupert
Hufnagel, Petra
Penzel, Roland
Freitag, Jens
Ströbel, Philipp
Kern, Michael A
Schröder, Sören
Neuhold, Nikolaus
Schmid, Kurt W
Schirmacher, Peter
Hartmann, Arndt
Rieker, Ralf J
author_facet Veits, Lothar
Schupfner, Rupert
Hufnagel, Petra
Penzel, Roland
Freitag, Jens
Ströbel, Philipp
Kern, Michael A
Schröder, Sören
Neuhold, Nikolaus
Schmid, Kurt W
Schirmacher, Peter
Hartmann, Arndt
Rieker, Ralf J
author_sort Veits, Lothar
collection PubMed
description BACKGROUND: CASTLE (Carcinoma showing thymus-like elements) is a rare malignant neoplasm of the thyroid resembling lymphoepithelioma-like and squamous cell carcinoma of the thymus with different biological behaviour and a better prognosis than anaplastic carcinoma of the thyroid. METHODS: We retrospectively investigated 6 cases of this very rare neoplasm in order to investigate the mutational status of KRAS, EGFR, PDGFR-α and KIT, as well as the immunohistochemical expression pattern of CD117, EGFR and COX-2, and possibly find new therapeutic targets. RESULTS: Diagnosis was confirmed by a moderate to strong expression of CD5, CD117 and CK5/6, whereas thyroglobulin, calcitonin and TTF-1 were negative in all cases. Tumors were also positive for COX-2 and in nearly all cases for EGFR. In four cases single nucleotide polymorphisms (SNPs) could be detected in exon 12 of the PDGFR-α gene (rs1873778), in three cases SNPs were found in exon 20 of the EGFR gene (rs1050171). No mutations were found in the KIT and KRAS gene. CONCLUSIONS: All tumors showed a COX-2 expression as well as an EGFR expression except for one case and a wild-type KRAS status. No activating mutations in the EGFR, KIT and PDGFR-α gene could be detected. Our data may indicate a potential for targeted therapies, but if these therapeutic strategies are of benefit in CASTLE remains to be determined. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1658499296115016
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spelling pubmed-40789822014-07-03 KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE) Veits, Lothar Schupfner, Rupert Hufnagel, Petra Penzel, Roland Freitag, Jens Ströbel, Philipp Kern, Michael A Schröder, Sören Neuhold, Nikolaus Schmid, Kurt W Schirmacher, Peter Hartmann, Arndt Rieker, Ralf J Diagn Pathol Research BACKGROUND: CASTLE (Carcinoma showing thymus-like elements) is a rare malignant neoplasm of the thyroid resembling lymphoepithelioma-like and squamous cell carcinoma of the thymus with different biological behaviour and a better prognosis than anaplastic carcinoma of the thyroid. METHODS: We retrospectively investigated 6 cases of this very rare neoplasm in order to investigate the mutational status of KRAS, EGFR, PDGFR-α and KIT, as well as the immunohistochemical expression pattern of CD117, EGFR and COX-2, and possibly find new therapeutic targets. RESULTS: Diagnosis was confirmed by a moderate to strong expression of CD5, CD117 and CK5/6, whereas thyroglobulin, calcitonin and TTF-1 were negative in all cases. Tumors were also positive for COX-2 and in nearly all cases for EGFR. In four cases single nucleotide polymorphisms (SNPs) could be detected in exon 12 of the PDGFR-α gene (rs1873778), in three cases SNPs were found in exon 20 of the EGFR gene (rs1050171). No mutations were found in the KIT and KRAS gene. CONCLUSIONS: All tumors showed a COX-2 expression as well as an EGFR expression except for one case and a wild-type KRAS status. No activating mutations in the EGFR, KIT and PDGFR-α gene could be detected. Our data may indicate a potential for targeted therapies, but if these therapeutic strategies are of benefit in CASTLE remains to be determined. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1658499296115016 BioMed Central 2014-06-16 /pmc/articles/PMC4078982/ /pubmed/24934485 http://dx.doi.org/10.1186/1746-1596-9-116 Text en Copyright © 2014 Veits et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Veits, Lothar
Schupfner, Rupert
Hufnagel, Petra
Penzel, Roland
Freitag, Jens
Ströbel, Philipp
Kern, Michael A
Schröder, Sören
Neuhold, Nikolaus
Schmid, Kurt W
Schirmacher, Peter
Hartmann, Arndt
Rieker, Ralf J
KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE)
title KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE)
title_full KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE)
title_fullStr KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE)
title_full_unstemmed KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE)
title_short KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE)
title_sort kras, egfr, pdgfr-α, kit and cox-2 status in carcinoma showing thymus-like elements (castle)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078982/
https://www.ncbi.nlm.nih.gov/pubmed/24934485
http://dx.doi.org/10.1186/1746-1596-9-116
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