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Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke
BACKGROUND: The brain endothelium is a key component of the blood brain barrier which is compromised following ischemia, allowing infiltration of damaging immune cells and other inflammatory molecules into the brain. Intravenous immunoglobulin (IVIg) is known to reduce infarct size in a mouse model...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079166/ https://www.ncbi.nlm.nih.gov/pubmed/24991401 http://dx.doi.org/10.1186/2040-7378-6-7 |
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author | Widiapradja, Alexander Santro, Tomislav Basta, Milan Sobey, Christopher G Manzanero, Silvia Arumugam, Thiruma V |
author_facet | Widiapradja, Alexander Santro, Tomislav Basta, Milan Sobey, Christopher G Manzanero, Silvia Arumugam, Thiruma V |
author_sort | Widiapradja, Alexander |
collection | PubMed |
description | BACKGROUND: The brain endothelium is a key component of the blood brain barrier which is compromised following ischemia, allowing infiltration of damaging immune cells and other inflammatory molecules into the brain. Intravenous immunoglobulin (IVIg) is known to reduce infarct size in a mouse model of experimental stroke. FINDINGS: Flow cytometry analysis showed that the protective effect of IVIg in ischemia and reperfusion injury in vivo is associated with reduced leukocyte infiltration, suggesting an involvement of the endothelium. In an in vitro model of ischemia, permeability analysis of the mouse brain endothelial cell line bEnd.3 revealed that IVIg prevented the loss of permeability caused by oxygen and glucose deprivation (OGD). In addition, western blot analysis of these brain endothelial cells showed that IVIg prevented the down-regulation of tight junction proteins claudin 5 and occludin and the decline in anti-apoptotic proteins Bcl-2 and Bcl-XL caused by OGD. CONCLUSION: IVIg protects endothelial cells from ischemic insult. These studies support the use of IVIg as a pharmacological intervention for stroke therapy. |
format | Online Article Text |
id | pubmed-4079166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40791662014-07-03 Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke Widiapradja, Alexander Santro, Tomislav Basta, Milan Sobey, Christopher G Manzanero, Silvia Arumugam, Thiruma V Exp Transl Stroke Med Short Report BACKGROUND: The brain endothelium is a key component of the blood brain barrier which is compromised following ischemia, allowing infiltration of damaging immune cells and other inflammatory molecules into the brain. Intravenous immunoglobulin (IVIg) is known to reduce infarct size in a mouse model of experimental stroke. FINDINGS: Flow cytometry analysis showed that the protective effect of IVIg in ischemia and reperfusion injury in vivo is associated with reduced leukocyte infiltration, suggesting an involvement of the endothelium. In an in vitro model of ischemia, permeability analysis of the mouse brain endothelial cell line bEnd.3 revealed that IVIg prevented the loss of permeability caused by oxygen and glucose deprivation (OGD). In addition, western blot analysis of these brain endothelial cells showed that IVIg prevented the down-regulation of tight junction proteins claudin 5 and occludin and the decline in anti-apoptotic proteins Bcl-2 and Bcl-XL caused by OGD. CONCLUSION: IVIg protects endothelial cells from ischemic insult. These studies support the use of IVIg as a pharmacological intervention for stroke therapy. BioMed Central 2014-06-20 /pmc/articles/PMC4079166/ /pubmed/24991401 http://dx.doi.org/10.1186/2040-7378-6-7 Text en Copyright © 2014 Widiapradja et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Widiapradja, Alexander Santro, Tomislav Basta, Milan Sobey, Christopher G Manzanero, Silvia Arumugam, Thiruma V Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke |
title | Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke |
title_full | Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke |
title_fullStr | Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke |
title_full_unstemmed | Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke |
title_short | Intravenous immunoglobulin (IVIg) provides protection against endothelial cell dysfunction and death in ischemic stroke |
title_sort | intravenous immunoglobulin (ivig) provides protection against endothelial cell dysfunction and death in ischemic stroke |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079166/ https://www.ncbi.nlm.nih.gov/pubmed/24991401 http://dx.doi.org/10.1186/2040-7378-6-7 |
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