Cargando…
Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma
Saw palmetto extract can induce the apoptosis of prostate cancer cells. The aim of the present study was to investigate the effect of saw palmetto extract on the phosphatidylinositol 3-kinase (PI3K)/Akt signaling transduction pathway in human glioma U87 and U251 cell lines. Suspensions of U87 and U2...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079418/ https://www.ncbi.nlm.nih.gov/pubmed/25009620 http://dx.doi.org/10.3892/etm.2014.1756 |
_version_ | 1782323848350269440 |
---|---|
author | YANG, YANG HUI, LV YUQIN, CHE JIE, LI SHUAI, HOU TIEZHU, ZHOU WEI, WANG |
author_facet | YANG, YANG HUI, LV YUQIN, CHE JIE, LI SHUAI, HOU TIEZHU, ZHOU WEI, WANG |
author_sort | YANG, YANG |
collection | PubMed |
description | Saw palmetto extract can induce the apoptosis of prostate cancer cells. The aim of the present study was to investigate the effect of saw palmetto extract on the phosphatidylinositol 3-kinase (PI3K)/Akt signaling transduction pathway in human glioma U87 and U251 cell lines. Suspensions of U87 and U251 cells in a logarithmic growth phase were seeded into six-well plates at a density of 10(4) cells/well. In the experimental group, 1 μl/ml saw palmetto extract was added, while the control group was cultured without a drug for 24 h. The expression levels of PI3K, B-cell lymphoma-extra large (Bcl-xL) and p53 were evaluated through western blot analysis. In the experimental group, the U87 and U251 cells exhibited a lower expression level of PI3K protein as compared with the control group (t=6.849; P<0.001). In addition, the two cell lines had a higher expression level of p53 protein in the experimental group as compared with the control group (t=40.810; P<0.001). Protein expression levels of Bcl-xL decreased significantly in the experimental group as compared with the control group (t=19.640; P=0.000). Therefore, saw palmetto extract induces glioma cell growth arrest and apoptosis via decreasing PI3K/Akt signal transduction. |
format | Online Article Text |
id | pubmed-4079418 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-40794182014-07-09 Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma YANG, YANG HUI, LV YUQIN, CHE JIE, LI SHUAI, HOU TIEZHU, ZHOU WEI, WANG Exp Ther Med Articles Saw palmetto extract can induce the apoptosis of prostate cancer cells. The aim of the present study was to investigate the effect of saw palmetto extract on the phosphatidylinositol 3-kinase (PI3K)/Akt signaling transduction pathway in human glioma U87 and U251 cell lines. Suspensions of U87 and U251 cells in a logarithmic growth phase were seeded into six-well plates at a density of 10(4) cells/well. In the experimental group, 1 μl/ml saw palmetto extract was added, while the control group was cultured without a drug for 24 h. The expression levels of PI3K, B-cell lymphoma-extra large (Bcl-xL) and p53 were evaluated through western blot analysis. In the experimental group, the U87 and U251 cells exhibited a lower expression level of PI3K protein as compared with the control group (t=6.849; P<0.001). In addition, the two cell lines had a higher expression level of p53 protein in the experimental group as compared with the control group (t=40.810; P<0.001). Protein expression levels of Bcl-xL decreased significantly in the experimental group as compared with the control group (t=19.640; P=0.000). Therefore, saw palmetto extract induces glioma cell growth arrest and apoptosis via decreasing PI3K/Akt signal transduction. D.A. Spandidos 2014-08 2014-06-04 /pmc/articles/PMC4079418/ /pubmed/25009620 http://dx.doi.org/10.3892/etm.2014.1756 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles YANG, YANG HUI, LV YUQIN, CHE JIE, LI SHUAI, HOU TIEZHU, ZHOU WEI, WANG Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma |
title | Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma |
title_full | Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma |
title_fullStr | Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma |
title_full_unstemmed | Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma |
title_short | Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma |
title_sort | effect of saw palmetto extract on pi3k cell signaling transduction in human glioma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079418/ https://www.ncbi.nlm.nih.gov/pubmed/25009620 http://dx.doi.org/10.3892/etm.2014.1756 |
work_keys_str_mv | AT yangyang effectofsawpalmettoextractonpi3kcellsignalingtransductioninhumanglioma AT huilv effectofsawpalmettoextractonpi3kcellsignalingtransductioninhumanglioma AT yuqinche effectofsawpalmettoextractonpi3kcellsignalingtransductioninhumanglioma AT jieli effectofsawpalmettoextractonpi3kcellsignalingtransductioninhumanglioma AT shuaihou effectofsawpalmettoextractonpi3kcellsignalingtransductioninhumanglioma AT tiezhuzhou effectofsawpalmettoextractonpi3kcellsignalingtransductioninhumanglioma AT weiwang effectofsawpalmettoextractonpi3kcellsignalingtransductioninhumanglioma |