Cargando…

Construction and verification of the targeted uPA-shRNA lentiviral vector and evaluation of the transfection and silencing rate

Urokinase-type plasminogen activator (uPA) receptors, which are released by the synovial tissue, are responsible for the activation of cartilage-breakdown proteases and play critical roles in cartilage degradation during the progression of osteoarthritis (OA). RNA interference (RNAi) technology has...

Descripción completa

Detalles Bibliográficos
Autores principales:	WANG, WEI-SHAN, GUO, FENG-JING, LI, CHANG-JUN, ZHANG, ZHEN-DONG, SHI, CHEN-HUI
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	D.A. Spandidos 2014
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079424/ https://www.ncbi.nlm.nih.gov/pubmed/25009597 http://dx.doi.org/10.3892/etm.2014.1741

Ejemplares similares

Construction of shRNA lentiviral vector
por: Song, Hong, et al.
Publicado: (2010)

Construction of a Single Lentiviral Vector Containing Tetracycline-Inducible Alb-uPA for Transduction of uPA Expression in Murine Hepatocytes
por: Bai, Jiasi, et al.
Publicado: (2013)

TEM8 functions as a receptor for uPA and mediates uPA-stimulated EGFR phosphorylation
por: Zhang, Lian-Cheng, et al.
Publicado: (2018)

A Comparison of Target Gene Silencing using Synthetically Modified siRNA and shRNA That Express Recombinant Lentiviral Vectors
por: Spirin, P.V., et al.
Publicado: (2009)

Lentiviral Vector Design for Multiple shRNA Expression and Durable HIV-1 Inhibition
por: Brake, Olivier ter, et al.
Publicado: (2008)

Construction of a lentiviral vector containing shRNA targeting ADAM17 and its role in attenuating endotoxemia in mice
por: He, Bing, et al.
Publicado: (2017)

Construction of a lentiviral vector containing shRNA targeting ADAM17 and its role in attenuating endotoxemia in mice
por: He, Bing, et al.
Publicado: (2018)

A new design of a lentiviral shRNA vector with inducible co-expression of ARGONAUTE 2 for enhancing gene silencing efficiency
por: He, Jiening, et al.
Publicado: (2015)

Independent validation of stromal uPA in ABCSG-08: Level 1b evidence for the prognostic value of uPA immunohistochemistry
por: Singer, C.F., et al.
Publicado: (2022)

Efficient shRNA delivery into B and T lymphoma cells using lentiviral vector-mediated transfer
por: Anastasov, Nataša, et al.
Publicado: (2008)

Design and cloning strategies for constructing shRNA expression vectors
por: McIntyre, Glen J, et al.
Publicado: (2006)

The uPA/uPAR system in astrocytic wound healing
por: Yepes, Manuel
Publicado: (2022)

Cassette deletion in multiple shRNA lentiviral vectors for HIV-1 and its impact on treatment success
por: Mcintyre, Glen J, et al.
Publicado: (2009)

uPA-mediated plasminogen activation is enhanced by polyphosphate
por: Whyte, Claire S., et al.
Publicado: (2020)

Criteria for effective design, construction, and gene knockdown by shRNA vectors
por: Taxman, Debra J, et al.
Publicado: (2006)

Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves
por: Kochtebane, Najlah, et al.
Publicado: (2014)

uPA deficiency exacerbates muscular dystrophy in MDX mice
por: Suelves, Mònica, et al.
Publicado: (2007)

uPA deficiency exacerbates muscular dystrophy in MDX mice
por: Suelves, Mònica, et al.
Publicado: (2007)

COVID-19 and pneumonia: a role for the uPA/uPAR system
por: D’Alonzo, Daniele, et al.
Publicado: (2020)

A Method to Assess Target Gene Involvement in Angiogenesis In Vitro and In Vivo Using Lentiviral Vectors Expressing shRNA
por: Blosser, Wayne, et al.
Publicado: (2014)

Clinical significance of the uPA system in gastric cancer with peritoneal metastasis
por: Ding, Youcheng, et al.
Publicado: (2013)

uPA dependent and independent mechanisms of wound healing by C-phycocyanin
por: Madhyastha, H K, et al.
Publicado: (2008)

Inhibition of Human Urokinase-Type Plasminogen Activator (uPA) Enzyme Activity and Receptor Binding by DNA Aptamers as Potential Therapeutics through Binding to the Different Forms of uPA
por: Dreymann, Nico, et al.
Publicado: (2022)

Silencing Human Rb2/p130 with shRNA
por: Leucci, Eleonora, et al.
Publicado: (2007)

Multiplex shRNA Screening of Germ Cell Development by in Vivo Transfection of Mouse Testis
por: Ho, Nicholas R. Y., et al.
Publicado: (2016)

A multi-shRNA vector enhances the silencing efficiency of exogenous and endogenous genes in human cells
por: Weng, Yanjie, et al.
Publicado: (2017)

RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs
por: Olson, A., et al.
Publicado: (2006)

Silencing of ENO1 by shRNA Inhibits the Proliferation of Gastric Cancer Cells
por: Qiao, Hui, et al.
Publicado: (2018)

A streamlined sub-cloning procedure to transfer shRNA from a pSM2 vector to a pGIPZ lentiviral vector
por: Ansaloni, Sara, et al.
Publicado: (2010)

Poised Transcription Factories Prime Silent uPA Gene Prior to Activation
por: Ferrai, Carmelo, et al.
Publicado: (2010)

The autoactivation of human single-chain urokinase-type plasminogen activator (uPA)
por: Torres-Paris, Constanza, et al.
Publicado: (2023)

Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK
por: Luo, Jie, et al.
Publicado: (2011)

Antibody-based PET of uPA/uPAR signaling with broad applicability for cancer imaging
por: Yang, Dongzhi, et al.
Publicado: (2016)

Prognostic significance of uPA and uPAR expression in patients with cervical cancer undergoing radiotherapy
por: Nantajit, Danupon, et al.
Publicado: (2021)

Targeting the Regulatory Subunit R2Alpha of Protein Kinase A in Human Glioblastoma through shRNA-Expressing Lentiviral Vectors
por: Zorzan, Maira, et al.
Publicado: (2021)

Cationic vesicles for efficient shRNA transfection in the MCF-7 breast cancer cell line
por: Mokhtary, Pardis, et al.
Publicado: (2018)

Downregulation of mouse CCR3 by lentiviral shRNA inhibits proliferation and induces apoptosis of mouse eosinophils
por: Zhu, Xin-Hua, et al.
Publicado: (2017)

Suppression of uPA and uPAR Attenuates Angiogenin Mediated Angiogenesis in Endothelial and Glioblastoma Cell Lines
por: Raghu, Hari, et al.
Publicado: (2010)

Optimization and characterization of tRNA-shRNA expression constructs
por: Scherer, Lisa J., et al.
Publicado: (2007)

Microglia and the urokinase plasminogen activator receptor/uPA system in innate brain inflammation
por: Cunningham, Orla, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_d94pt87o6albh6d11bcrh9ekme