Cargando…
Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study
BACKGROUND: Highly abundant proteins in biological fluids such as serum or cerebrospinal fluid (CSF) can hinder the detection of proteins in lower abundance, e.g., potential biomarkers. Commercial products are available for the depletion of albumin and immunoglobulins (Igs), although most of these k...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079625/ https://www.ncbi.nlm.nih.gov/pubmed/25002965 http://dx.doi.org/10.1186/2045-8118-11-14 |
_version_ | 1782323876878876672 |
---|---|
author | Günther, Ramona Krause, Eberhard Schümann, Michael Ausseil, Jérome Heard, Jean-Michel Blasig, Ingolf E Haseloff, Reiner F |
author_facet | Günther, Ramona Krause, Eberhard Schümann, Michael Ausseil, Jérome Heard, Jean-Michel Blasig, Ingolf E Haseloff, Reiner F |
author_sort | Günther, Ramona |
collection | PubMed |
description | BACKGROUND: Highly abundant proteins in biological fluids such as serum or cerebrospinal fluid (CSF) can hinder the detection of proteins in lower abundance, e.g., potential biomarkers. Commercial products are available for the depletion of albumin and immunoglobulins (Igs), although most of these kits have not been validated for dog samples. The present study therefore examines the use of different types of depletion kits for dog CSF. FINDINGS: Three kits, with different mechanisms for the depletion of albumin and Igs, were tested with dog CSF specimens. One product significantly decreased the amount of albumin; with all kits, IgG was less efficiently removed than albumin. Mass spectrometry of the fractions eluted from the depletion columns revealed considerable co-depletion of other CSF proteins. CONCLUSIONS: A commercially available depletion kit was identified which depletes albumin and (to a lower extent) immunoglobulins from dog CSF. However, the limited efficacy and the concomitant loss of other proteins from the sample should be taken into account when using this product. |
format | Online Article Text |
id | pubmed-4079625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40796252014-07-07 Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study Günther, Ramona Krause, Eberhard Schümann, Michael Ausseil, Jérome Heard, Jean-Michel Blasig, Ingolf E Haseloff, Reiner F Fluids Barriers CNS Short Paper BACKGROUND: Highly abundant proteins in biological fluids such as serum or cerebrospinal fluid (CSF) can hinder the detection of proteins in lower abundance, e.g., potential biomarkers. Commercial products are available for the depletion of albumin and immunoglobulins (Igs), although most of these kits have not been validated for dog samples. The present study therefore examines the use of different types of depletion kits for dog CSF. FINDINGS: Three kits, with different mechanisms for the depletion of albumin and Igs, were tested with dog CSF specimens. One product significantly decreased the amount of albumin; with all kits, IgG was less efficiently removed than albumin. Mass spectrometry of the fractions eluted from the depletion columns revealed considerable co-depletion of other CSF proteins. CONCLUSIONS: A commercially available depletion kit was identified which depletes albumin and (to a lower extent) immunoglobulins from dog CSF. However, the limited efficacy and the concomitant loss of other proteins from the sample should be taken into account when using this product. BioMed Central 2014-06-23 /pmc/articles/PMC4079625/ /pubmed/25002965 http://dx.doi.org/10.1186/2045-8118-11-14 Text en Copyright © 2014 Günther et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Paper Günther, Ramona Krause, Eberhard Schümann, Michael Ausseil, Jérome Heard, Jean-Michel Blasig, Ingolf E Haseloff, Reiner F Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study |
title | Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study |
title_full | Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study |
title_fullStr | Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study |
title_full_unstemmed | Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study |
title_short | Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study |
title_sort | removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study |
topic | Short Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079625/ https://www.ncbi.nlm.nih.gov/pubmed/25002965 http://dx.doi.org/10.1186/2045-8118-11-14 |
work_keys_str_mv | AT guntherramona removalofalbuminandimmunoglobulinsfromcaninecerebrospinalfluidusingdepletionkitsafeasibilitystudy AT krauseeberhard removalofalbuminandimmunoglobulinsfromcaninecerebrospinalfluidusingdepletionkitsafeasibilitystudy AT schumannmichael removalofalbuminandimmunoglobulinsfromcaninecerebrospinalfluidusingdepletionkitsafeasibilitystudy AT ausseiljerome removalofalbuminandimmunoglobulinsfromcaninecerebrospinalfluidusingdepletionkitsafeasibilitystudy AT heardjeanmichel removalofalbuminandimmunoglobulinsfromcaninecerebrospinalfluidusingdepletionkitsafeasibilitystudy AT blasigingolfe removalofalbuminandimmunoglobulinsfromcaninecerebrospinalfluidusingdepletionkitsafeasibilitystudy AT haseloffreinerf removalofalbuminandimmunoglobulinsfromcaninecerebrospinalfluidusingdepletionkitsafeasibilitystudy |