Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability

Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer...

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Detalles Bibliográficos
Autores principales: Terracciano, Monica, Rea, Ilaria, De Stefano, Luca, Rendina, Ivo, Oliviero, Giorgia, Nici, Fabrizia, D'Errico, Stefano, Piccialli, Gennaro, Borbone, Nicola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Nano Express
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079915/
https://www.ncbi.nlm.nih.gov/pubmed/25114630
http://dx.doi.org/10.1186/1556-276X-9-317
Descripción
Sumario:Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer mixed sequence has been successfully synthesized on aminosilane-modified porous silicon photonic structures. The process and the materials have been characterized by optical reflectivity, atomic force microscopy and high-performance liquid chromatography.

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