Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability

Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer...

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Autores principales: Terracciano, Monica, Rea, Ilaria, De Stefano, Luca, Rendina, Ivo, Oliviero, Giorgia, Nici, Fabrizia, D'Errico, Stefano, Piccialli, Gennaro, Borbone, Nicola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Nano Express
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079915/
https://www.ncbi.nlm.nih.gov/pubmed/25114630
http://dx.doi.org/10.1186/1556-276X-9-317
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author Terracciano, Monica
Rea, Ilaria
De Stefano, Luca
Rendina, Ivo
Oliviero, Giorgia
Nici, Fabrizia
D'Errico, Stefano
Piccialli, Gennaro
Borbone, Nicola
author_facet Terracciano, Monica
Rea, Ilaria
De Stefano, Luca
Rendina, Ivo
Oliviero, Giorgia
Nici, Fabrizia
D'Errico, Stefano
Piccialli, Gennaro
Borbone, Nicola
author_sort Terracciano, Monica
collection PubMed
description Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer mixed sequence has been successfully synthesized on aminosilane-modified porous silicon photonic structures. The process and the materials have been characterized by optical reflectivity, atomic force microscopy and high-performance liquid chromatography.
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spelling pubmed-40799152014-08-11 Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability Terracciano, Monica Rea, Ilaria De Stefano, Luca Rendina, Ivo Oliviero, Giorgia Nici, Fabrizia D'Errico, Stefano Piccialli, Gennaro Borbone, Nicola Nanoscale Res Lett Nano Express Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer mixed sequence has been successfully synthesized on aminosilane-modified porous silicon photonic structures. The process and the materials have been characterized by optical reflectivity, atomic force microscopy and high-performance liquid chromatography. Springer 2014-06-25 /pmc/articles/PMC4079915/ /pubmed/25114630 http://dx.doi.org/10.1186/1556-276X-9-317 Text en Copyright © 2014 Terracciano et al.; licensee Springer. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Nano Express
Terracciano, Monica
Rea, Ilaria
De Stefano, Luca
Rendina, Ivo
Oliviero, Giorgia
Nici, Fabrizia
D'Errico, Stefano
Piccialli, Gennaro
Borbone, Nicola
Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability
title Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability
title_full Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability
title_fullStr Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability
title_full_unstemmed Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability
title_short Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability
title_sort synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079915/
https://www.ncbi.nlm.nih.gov/pubmed/25114630
http://dx.doi.org/10.1186/1556-276X-9-317
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