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Identification of MHCII variants associated with chlamydial disease in the koala (Phascolarctos cinereus)

Chlamydiosis, the most common infectious disease in koalas, can cause chronic urogenital tract fibrosis and infertility. High titres of serum immunoglobulin G against 10 kDa and 60 kDa chlamydial heat-shock proteins (c-hsp10 and c-hsp60) are associated with fibrous occlusion of the koala uterus and...

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Autores principales: Lau, Quintin, Griffith, Joanna E., Higgins, Damien P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081129/
https://www.ncbi.nlm.nih.gov/pubmed/25024912
http://dx.doi.org/10.7717/peerj.443
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author Lau, Quintin
Griffith, Joanna E.
Higgins, Damien P.
author_facet Lau, Quintin
Griffith, Joanna E.
Higgins, Damien P.
author_sort Lau, Quintin
collection PubMed
description Chlamydiosis, the most common infectious disease in koalas, can cause chronic urogenital tract fibrosis and infertility. High titres of serum immunoglobulin G against 10 kDa and 60 kDa chlamydial heat-shock proteins (c-hsp10 and c-hsp60) are associated with fibrous occlusion of the koala uterus and uterine tube. Murine and human studies have identified associations between specific major histocompatibility complex class II (MHCII) alleles or genotypes, and higher c-hsp 60 antibody levels or chlamydia-associated disease and infertility. In this study, we characterised partial MHCII DAB and DBB genes in female koalas (n = 94) from a single geographic population, and investigated associations among antibody responses to c-hsp60 quantified by ELISA, susceptibility to chlamydial infection, or age. The identification of three candidate MHCII variants provides additional support for the functional role of MHCII in the koala, and will inform more focused future studies. This is the first study to investigate an association between MHC genes with chlamydial pathogenesis in a non-model, free-ranging species.
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spelling pubmed-40811292014-07-14 Identification of MHCII variants associated with chlamydial disease in the koala (Phascolarctos cinereus) Lau, Quintin Griffith, Joanna E. Higgins, Damien P. PeerJ Conservation Biology Chlamydiosis, the most common infectious disease in koalas, can cause chronic urogenital tract fibrosis and infertility. High titres of serum immunoglobulin G against 10 kDa and 60 kDa chlamydial heat-shock proteins (c-hsp10 and c-hsp60) are associated with fibrous occlusion of the koala uterus and uterine tube. Murine and human studies have identified associations between specific major histocompatibility complex class II (MHCII) alleles or genotypes, and higher c-hsp 60 antibody levels or chlamydia-associated disease and infertility. In this study, we characterised partial MHCII DAB and DBB genes in female koalas (n = 94) from a single geographic population, and investigated associations among antibody responses to c-hsp60 quantified by ELISA, susceptibility to chlamydial infection, or age. The identification of three candidate MHCII variants provides additional support for the functional role of MHCII in the koala, and will inform more focused future studies. This is the first study to investigate an association between MHC genes with chlamydial pathogenesis in a non-model, free-ranging species. PeerJ Inc. 2014-06-19 /pmc/articles/PMC4081129/ /pubmed/25024912 http://dx.doi.org/10.7717/peerj.443 Text en © 2014 Lau et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Conservation Biology
Lau, Quintin
Griffith, Joanna E.
Higgins, Damien P.
Identification of MHCII variants associated with chlamydial disease in the koala (Phascolarctos cinereus)
title Identification of MHCII variants associated with chlamydial disease in the koala (Phascolarctos cinereus)
title_full Identification of MHCII variants associated with chlamydial disease in the koala (Phascolarctos cinereus)
title_fullStr Identification of MHCII variants associated with chlamydial disease in the koala (Phascolarctos cinereus)
title_full_unstemmed Identification of MHCII variants associated with chlamydial disease in the koala (Phascolarctos cinereus)
title_short Identification of MHCII variants associated with chlamydial disease in the koala (Phascolarctos cinereus)
title_sort identification of mhcii variants associated with chlamydial disease in the koala (phascolarctos cinereus)
topic Conservation Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081129/
https://www.ncbi.nlm.nih.gov/pubmed/25024912
http://dx.doi.org/10.7717/peerj.443
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