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Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer

In order to investigate the pathogenic mechanisms of parvovirus B19 in human colorectal cancer, plasmids containing the VP1 or VP2 viral capsid proteins or the NS1 non-structural proteins of parvovirus B19 were constructed and transfected into primary human colorectal epithelial cells and LoVo cells...

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Autores principales: ZHANG, WEI-PING, YANG, HUA, CHEN, HONG, ZHU, HAI-RONG, LEI, QUAN, SONG, YUN-HONG, DAI, ZHONG-MING, SUN, JING-SHAN, JIANG, LI-LI, NIE, ZHAN-GUO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081382/
https://www.ncbi.nlm.nih.gov/pubmed/25013465
http://dx.doi.org/10.3892/ol.2014.2151
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author ZHANG, WEI-PING
YANG, HUA
CHEN, HONG
ZHU, HAI-RONG
LEI, QUAN
SONG, YUN-HONG
DAI, ZHONG-MING
SUN, JING-SHAN
JIANG, LI-LI
NIE, ZHAN-GUO
author_facet ZHANG, WEI-PING
YANG, HUA
CHEN, HONG
ZHU, HAI-RONG
LEI, QUAN
SONG, YUN-HONG
DAI, ZHONG-MING
SUN, JING-SHAN
JIANG, LI-LI
NIE, ZHAN-GUO
author_sort ZHANG, WEI-PING
collection PubMed
description In order to investigate the pathogenic mechanisms of parvovirus B19 in human colorectal cancer, plasmids containing the VP1 or VP2 viral capsid proteins or the NS1 non-structural proteins of parvovirus B19 were constructed and transfected into primary human colorectal epithelial cells and LoVo cells. Differential gene expression was detected using a human genome expression array. Functional gene annotation analyses were performed using Database for Annotation, Visualization and Integrated Discovery v6.7 software. Gene ontology (GO) analyses revealed that VP1-related functions included the immune response, immune system process, defense response and the response to stimulus, while NS1-associated functions were found to include organelle fission, nuclear division, mitosis, the M-phase of the mitotic cell cycle, the mitotic cell cycle, M-phase, cell cycle phase, cell cycle process and cell division. Pathway expression analysis revealed that VP1-associated pathways included cell adhesion molecules, antigen processing and presentation, cytokines and the inflammatory response. Moreover, NS1-associated pathways included the cell cycle, pathways in cancer, colorectal cancer, the wnt signaling pathway and focal adhesion. Among the differential genes detected in the present study, 12 genes were found to participate in general cancer pathways and six genes were observed to participate in colorectal cancer pathways. NS1 is a key molecule in the pathogenic mechanism of parvovirus B19 in colorectal cancer. Several GO categories, pathways and genes were selected and may be the key targets through which parvovirus B19 participates in colorectal cancer pathogenesis.
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spelling pubmed-40813822014-07-10 Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer ZHANG, WEI-PING YANG, HUA CHEN, HONG ZHU, HAI-RONG LEI, QUAN SONG, YUN-HONG DAI, ZHONG-MING SUN, JING-SHAN JIANG, LI-LI NIE, ZHAN-GUO Oncol Lett Articles In order to investigate the pathogenic mechanisms of parvovirus B19 in human colorectal cancer, plasmids containing the VP1 or VP2 viral capsid proteins or the NS1 non-structural proteins of parvovirus B19 were constructed and transfected into primary human colorectal epithelial cells and LoVo cells. Differential gene expression was detected using a human genome expression array. Functional gene annotation analyses were performed using Database for Annotation, Visualization and Integrated Discovery v6.7 software. Gene ontology (GO) analyses revealed that VP1-related functions included the immune response, immune system process, defense response and the response to stimulus, while NS1-associated functions were found to include organelle fission, nuclear division, mitosis, the M-phase of the mitotic cell cycle, the mitotic cell cycle, M-phase, cell cycle phase, cell cycle process and cell division. Pathway expression analysis revealed that VP1-associated pathways included cell adhesion molecules, antigen processing and presentation, cytokines and the inflammatory response. Moreover, NS1-associated pathways included the cell cycle, pathways in cancer, colorectal cancer, the wnt signaling pathway and focal adhesion. Among the differential genes detected in the present study, 12 genes were found to participate in general cancer pathways and six genes were observed to participate in colorectal cancer pathways. NS1 is a key molecule in the pathogenic mechanism of parvovirus B19 in colorectal cancer. Several GO categories, pathways and genes were selected and may be the key targets through which parvovirus B19 participates in colorectal cancer pathogenesis. D.A. Spandidos 2014-08 2014-05-16 /pmc/articles/PMC4081382/ /pubmed/25013465 http://dx.doi.org/10.3892/ol.2014.2151 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ZHANG, WEI-PING
YANG, HUA
CHEN, HONG
ZHU, HAI-RONG
LEI, QUAN
SONG, YUN-HONG
DAI, ZHONG-MING
SUN, JING-SHAN
JIANG, LI-LI
NIE, ZHAN-GUO
Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
title Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
title_full Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
title_fullStr Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
title_full_unstemmed Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
title_short Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
title_sort gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus b19 in human colorectal cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081382/
https://www.ncbi.nlm.nih.gov/pubmed/25013465
http://dx.doi.org/10.3892/ol.2014.2151
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