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Quercetin induces HepG2 cell apoptosis by inhibiting fatty acid biosynthesis

Quercetin can inhibit the growth of cancer cells with the ability to act as a ‘chemopreventer’. Its cancer-preventive effect has been attributed to various mechanisms, including the induction of cell-cycle arrest and/or apoptosis, as well as its antioxidant functions. Quercetin can also reduce adipo...

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Autores principales: ZHAO, PENG, MAO, JUN-MIN, ZHANG, SHU-YUN, ZHOU, ZE-QUAN, TAN, YANG, ZHANG, YU
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081423/
https://www.ncbi.nlm.nih.gov/pubmed/25009654
http://dx.doi.org/10.3892/ol.2014.2159
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author ZHAO, PENG
MAO, JUN-MIN
ZHANG, SHU-YUN
ZHOU, ZE-QUAN
TAN, YANG
ZHANG, YU
author_facet ZHAO, PENG
MAO, JUN-MIN
ZHANG, SHU-YUN
ZHOU, ZE-QUAN
TAN, YANG
ZHANG, YU
author_sort ZHAO, PENG
collection PubMed
description Quercetin can inhibit the growth of cancer cells with the ability to act as a ‘chemopreventer’. Its cancer-preventive effect has been attributed to various mechanisms, including the induction of cell-cycle arrest and/or apoptosis, as well as its antioxidant functions. Quercetin can also reduce adipogenesis. Previous studies have shown that quercetin has potent inhibitory effects on animal fatty acid synthase (FASN). In the present study, activity of quercetin was evaluated in human liver cancer HepG2 cells. Intracellular FASN activity was calculated by measuring the absorption of NADPH via a spectrophotometer. MTT assay was used to test the cell viability, immunoblot analysis was performed to detect FASN expression levels and the apoptotic effect was detected by Hoechst 33258 staining. In the present study, it was found that quercetin could induce apoptosis in human liver cancer HepG2 cells with overexpression of FASN. This apoptosis was accompanied by the reduction of intracellular FASN activity and could be rescued by 25 or 50 μM exogenous palmitic acids, the final product of FASN-catalyzed synthesis. These results suggested that the apoptosis induced by quercetin was via the inhibition of FASN. These findings suggested that quercetin may be useful for preventing human liver cancer.
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spelling pubmed-40814232014-07-09 Quercetin induces HepG2 cell apoptosis by inhibiting fatty acid biosynthesis ZHAO, PENG MAO, JUN-MIN ZHANG, SHU-YUN ZHOU, ZE-QUAN TAN, YANG ZHANG, YU Oncol Lett Articles Quercetin can inhibit the growth of cancer cells with the ability to act as a ‘chemopreventer’. Its cancer-preventive effect has been attributed to various mechanisms, including the induction of cell-cycle arrest and/or apoptosis, as well as its antioxidant functions. Quercetin can also reduce adipogenesis. Previous studies have shown that quercetin has potent inhibitory effects on animal fatty acid synthase (FASN). In the present study, activity of quercetin was evaluated in human liver cancer HepG2 cells. Intracellular FASN activity was calculated by measuring the absorption of NADPH via a spectrophotometer. MTT assay was used to test the cell viability, immunoblot analysis was performed to detect FASN expression levels and the apoptotic effect was detected by Hoechst 33258 staining. In the present study, it was found that quercetin could induce apoptosis in human liver cancer HepG2 cells with overexpression of FASN. This apoptosis was accompanied by the reduction of intracellular FASN activity and could be rescued by 25 or 50 μM exogenous palmitic acids, the final product of FASN-catalyzed synthesis. These results suggested that the apoptosis induced by quercetin was via the inhibition of FASN. These findings suggested that quercetin may be useful for preventing human liver cancer. D.A. Spandidos 2014-08 2014-05-19 /pmc/articles/PMC4081423/ /pubmed/25009654 http://dx.doi.org/10.3892/ol.2014.2159 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ZHAO, PENG
MAO, JUN-MIN
ZHANG, SHU-YUN
ZHOU, ZE-QUAN
TAN, YANG
ZHANG, YU
Quercetin induces HepG2 cell apoptosis by inhibiting fatty acid biosynthesis
title Quercetin induces HepG2 cell apoptosis by inhibiting fatty acid biosynthesis
title_full Quercetin induces HepG2 cell apoptosis by inhibiting fatty acid biosynthesis
title_fullStr Quercetin induces HepG2 cell apoptosis by inhibiting fatty acid biosynthesis
title_full_unstemmed Quercetin induces HepG2 cell apoptosis by inhibiting fatty acid biosynthesis
title_short Quercetin induces HepG2 cell apoptosis by inhibiting fatty acid biosynthesis
title_sort quercetin induces hepg2 cell apoptosis by inhibiting fatty acid biosynthesis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081423/
https://www.ncbi.nlm.nih.gov/pubmed/25009654
http://dx.doi.org/10.3892/ol.2014.2159
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