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Exome Sequencing from Nanogram Amounts of Starting DNA: Comparing Three Approaches
Hybridization-based target enrichment protocols require relatively large starting amounts of genomic DNA, which is not always available. Here, we tested three approaches to pre-capture library preparation starting from 10 ng of genomic DNA: (i and ii) whole-genome amplification of DNA samples with R...
Autores principales: | Rykalina, Vera N., Shadrin, Alexey A., Amstislavskiy, Vyacheslav S., Rogaev, Evgeny I., Lehrach, Hans, Borodina, Tatiana A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081514/ https://www.ncbi.nlm.nih.gov/pubmed/24992588 http://dx.doi.org/10.1371/journal.pone.0101154 |
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