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Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle
Bitter tastants can induce relaxation in precontracted airway smooth muscle by activating big-conductance potassium channels (BKs) or by inactivating voltage-dependent L-type Ca(2+) channels (VDLCCs). In this study, a new pathway for bitter tastant-induced relaxation was defined and investigated. We...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081631/ https://www.ncbi.nlm.nih.gov/pubmed/24992312 http://dx.doi.org/10.1371/journal.pone.0101578 |
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author | Zhang, Ting Luo, Xiao-Jing Sai, Wen-Bo Yu, Meng-Fei Li, Wen-Er Ma, Yun-Fei Chen, Weiwei Zhai, Kui Qin, Gangjian Guo, Donglin Zheng, Yun-Min Wang, Yong-Xiao Shen, Jin-Hua Ji, Guangju Liu, Qing-Hua |
author_facet | Zhang, Ting Luo, Xiao-Jing Sai, Wen-Bo Yu, Meng-Fei Li, Wen-Er Ma, Yun-Fei Chen, Weiwei Zhai, Kui Qin, Gangjian Guo, Donglin Zheng, Yun-Min Wang, Yong-Xiao Shen, Jin-Hua Ji, Guangju Liu, Qing-Hua |
author_sort | Zhang, Ting |
collection | PubMed |
description | Bitter tastants can induce relaxation in precontracted airway smooth muscle by activating big-conductance potassium channels (BKs) or by inactivating voltage-dependent L-type Ca(2+) channels (VDLCCs). In this study, a new pathway for bitter tastant-induced relaxation was defined and investigated. We found nifedipine-insensitive and bitter tastant chloroquine-sensitive relaxation in epithelium-denuded mouse tracheal rings (TRs) precontracted with acetylcholine (ACH). In the presence of nifedipine (10 µM), ACH induced cytosolic Ca(2+) elevation and cell shortening in single airway smooth muscle cells (ASMCs), and these changes were inhibited by chloroquine. In TRs, ACH triggered a transient contraction under Ca(2+)-free conditions, and, following a restoration of Ca(2+), a strong contraction occurred, which was inhibited by chloroquine. Moreover, the ACH-activated whole-cell and single channel currents of non-selective cation channels (NSCCs) were blocked by chloroquine. Pyrazole 3 (Pyr3), an inhibitor of transient receptor potential C3 (TRPC3) channels, partially inhibited ACH-induced contraction, intracellular Ca(2+) elevation, and NSCC currents. These results demonstrate that NSCCs play a role in bitter tastant-induced relaxation in precontracted airway smooth muscle. |
format | Online Article Text |
id | pubmed-4081631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40816312014-07-10 Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle Zhang, Ting Luo, Xiao-Jing Sai, Wen-Bo Yu, Meng-Fei Li, Wen-Er Ma, Yun-Fei Chen, Weiwei Zhai, Kui Qin, Gangjian Guo, Donglin Zheng, Yun-Min Wang, Yong-Xiao Shen, Jin-Hua Ji, Guangju Liu, Qing-Hua PLoS One Research Article Bitter tastants can induce relaxation in precontracted airway smooth muscle by activating big-conductance potassium channels (BKs) or by inactivating voltage-dependent L-type Ca(2+) channels (VDLCCs). In this study, a new pathway for bitter tastant-induced relaxation was defined and investigated. We found nifedipine-insensitive and bitter tastant chloroquine-sensitive relaxation in epithelium-denuded mouse tracheal rings (TRs) precontracted with acetylcholine (ACH). In the presence of nifedipine (10 µM), ACH induced cytosolic Ca(2+) elevation and cell shortening in single airway smooth muscle cells (ASMCs), and these changes were inhibited by chloroquine. In TRs, ACH triggered a transient contraction under Ca(2+)-free conditions, and, following a restoration of Ca(2+), a strong contraction occurred, which was inhibited by chloroquine. Moreover, the ACH-activated whole-cell and single channel currents of non-selective cation channels (NSCCs) were blocked by chloroquine. Pyrazole 3 (Pyr3), an inhibitor of transient receptor potential C3 (TRPC3) channels, partially inhibited ACH-induced contraction, intracellular Ca(2+) elevation, and NSCC currents. These results demonstrate that NSCCs play a role in bitter tastant-induced relaxation in precontracted airway smooth muscle. Public Library of Science 2014-07-03 /pmc/articles/PMC4081631/ /pubmed/24992312 http://dx.doi.org/10.1371/journal.pone.0101578 Text en © 2014 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhang, Ting Luo, Xiao-Jing Sai, Wen-Bo Yu, Meng-Fei Li, Wen-Er Ma, Yun-Fei Chen, Weiwei Zhai, Kui Qin, Gangjian Guo, Donglin Zheng, Yun-Min Wang, Yong-Xiao Shen, Jin-Hua Ji, Guangju Liu, Qing-Hua Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle |
title | Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle |
title_full | Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle |
title_fullStr | Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle |
title_full_unstemmed | Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle |
title_short | Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle |
title_sort | non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081631/ https://www.ncbi.nlm.nih.gov/pubmed/24992312 http://dx.doi.org/10.1371/journal.pone.0101578 |
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