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Monoaminergic control of spinal locomotor networks in SOD1(G93A) newborn mice

Mutations in the gene that encodes Cu/Zn-superoxide dismutase (SOD1) are the cause of approximately 20% of familial forms of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease characterized by the progressive loss of motor neurons. While ALS symptoms appear in adulthood, spinal m...

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Autores principales: Milan, Léa, Barrière, Grégory, De Deurwaerdère, Philippe, Cazalets, Jean-René, Bertrand, Sandrine S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081764/
https://www.ncbi.nlm.nih.gov/pubmed/25071458
http://dx.doi.org/10.3389/fncir.2014.00077
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author Milan, Léa
Barrière, Grégory
De Deurwaerdère, Philippe
Cazalets, Jean-René
Bertrand, Sandrine S.
author_facet Milan, Léa
Barrière, Grégory
De Deurwaerdère, Philippe
Cazalets, Jean-René
Bertrand, Sandrine S.
author_sort Milan, Léa
collection PubMed
description Mutations in the gene that encodes Cu/Zn-superoxide dismutase (SOD1) are the cause of approximately 20% of familial forms of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease characterized by the progressive loss of motor neurons. While ALS symptoms appear in adulthood, spinal motoneurons exhibit functional alterations as early as the embryonic and postnatal stages in the murine model of ALS, the SOD1 mice. Monoaminergic – i.e., dopaminergic (DA), serotoninergic (5-HT), and noradrenergic (NA) – pathways powerfully control spinal networks and contribute significantly to their embryonic and postnatal maturation. Alterations in monoaminergic neuromodulation during development could therefore lead to impairments in the motoneuronal physiology. In this study, we sought to determine whether the monoaminergic spinal systems are modified in the early stages of development in SOD1 mice. Using a post-mortem analysis by high performance liquid chromatography (HPLC), monoaminergic neuromodulators and their metabolites were quantified in the lumbar spinal cord of SOD1 and wild-type (WT) mice aged one postnatal day (P1) and P10. This analysis underscores an increased content of DA in the SOD1 lumbar spinal cord compared to that of WT mice but failed to reveal any modification of the other monoaminergic contents. In a next step, we compared the efficiency of the monoaminergic compounds in triggering and modulating fictive locomotion in WT and SOD1 mice. This study was performed in P1–P3 SOD1 mice and age-matched control littermates using extracellular recordings from the lumbar ventral roots in the in vitro isolated spinal cord preparation. This analysis revealed that the spinal networks of SOD1(G93A) mice could generate normal locomotor activity in the presence of NMA-5-HT. Interestingly, we also observed that SOD1 spinal networks have an increased sensitivity to NA compared to WT spinal circuits but exhibited similar DA responses.
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spelling pubmed-40817642014-07-28 Monoaminergic control of spinal locomotor networks in SOD1(G93A) newborn mice Milan, Léa Barrière, Grégory De Deurwaerdère, Philippe Cazalets, Jean-René Bertrand, Sandrine S. Front Neural Circuits Neuroscience Mutations in the gene that encodes Cu/Zn-superoxide dismutase (SOD1) are the cause of approximately 20% of familial forms of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease characterized by the progressive loss of motor neurons. While ALS symptoms appear in adulthood, spinal motoneurons exhibit functional alterations as early as the embryonic and postnatal stages in the murine model of ALS, the SOD1 mice. Monoaminergic – i.e., dopaminergic (DA), serotoninergic (5-HT), and noradrenergic (NA) – pathways powerfully control spinal networks and contribute significantly to their embryonic and postnatal maturation. Alterations in monoaminergic neuromodulation during development could therefore lead to impairments in the motoneuronal physiology. In this study, we sought to determine whether the monoaminergic spinal systems are modified in the early stages of development in SOD1 mice. Using a post-mortem analysis by high performance liquid chromatography (HPLC), monoaminergic neuromodulators and their metabolites were quantified in the lumbar spinal cord of SOD1 and wild-type (WT) mice aged one postnatal day (P1) and P10. This analysis underscores an increased content of DA in the SOD1 lumbar spinal cord compared to that of WT mice but failed to reveal any modification of the other monoaminergic contents. In a next step, we compared the efficiency of the monoaminergic compounds in triggering and modulating fictive locomotion in WT and SOD1 mice. This study was performed in P1–P3 SOD1 mice and age-matched control littermates using extracellular recordings from the lumbar ventral roots in the in vitro isolated spinal cord preparation. This analysis revealed that the spinal networks of SOD1(G93A) mice could generate normal locomotor activity in the presence of NMA-5-HT. Interestingly, we also observed that SOD1 spinal networks have an increased sensitivity to NA compared to WT spinal circuits but exhibited similar DA responses. Frontiers Media S.A. 2014-07-04 /pmc/articles/PMC4081764/ /pubmed/25071458 http://dx.doi.org/10.3389/fncir.2014.00077 Text en Copyright © 2014 Milan, Barrière, De Deurwaerdère, Cazalets and Bertrand. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Milan, Léa
Barrière, Grégory
De Deurwaerdère, Philippe
Cazalets, Jean-René
Bertrand, Sandrine S.
Monoaminergic control of spinal locomotor networks in SOD1(G93A) newborn mice
title Monoaminergic control of spinal locomotor networks in SOD1(G93A) newborn mice
title_full Monoaminergic control of spinal locomotor networks in SOD1(G93A) newborn mice
title_fullStr Monoaminergic control of spinal locomotor networks in SOD1(G93A) newborn mice
title_full_unstemmed Monoaminergic control of spinal locomotor networks in SOD1(G93A) newborn mice
title_short Monoaminergic control of spinal locomotor networks in SOD1(G93A) newborn mice
title_sort monoaminergic control of spinal locomotor networks in sod1(g93a) newborn mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081764/
https://www.ncbi.nlm.nih.gov/pubmed/25071458
http://dx.doi.org/10.3389/fncir.2014.00077
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