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Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

Cell surface glycoconjugates are used as markers for undifferentiated pluripotent stem cells. Here, antibody binding and mass spectrometry characterization of acid glycosphingolipids isolated from a large number (1 × 10(9) cells) of human embryonic stem cell (hESC) lines allowed identification of se...

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Autores principales: Barone, Angela, Säljö, Karin, Benktander, John, Blomqvist, Maria, Månsson, Jan-Eric, Johansson, Bengt R., Mölne, Johan, Aspegren, Anders, Björquist, Petter, Breimer, Michael E., Teneberg, Susann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081926/
https://www.ncbi.nlm.nih.gov/pubmed/24841197
http://dx.doi.org/10.1074/jbc.M114.568832
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author Barone, Angela
Säljö, Karin
Benktander, John
Blomqvist, Maria
Månsson, Jan-Eric
Johansson, Bengt R.
Mölne, Johan
Aspegren, Anders
Björquist, Petter
Breimer, Michael E.
Teneberg, Susann
author_facet Barone, Angela
Säljö, Karin
Benktander, John
Blomqvist, Maria
Månsson, Jan-Eric
Johansson, Bengt R.
Mölne, Johan
Aspegren, Anders
Björquist, Petter
Breimer, Michael E.
Teneberg, Susann
author_sort Barone, Angela
collection PubMed
description Cell surface glycoconjugates are used as markers for undifferentiated pluripotent stem cells. Here, antibody binding and mass spectrometry characterization of acid glycosphingolipids isolated from a large number (1 × 10(9) cells) of human embryonic stem cell (hESC) lines allowed identification of several novel acid glycosphingolipids, like the gangliosides sialyl-lactotetraosylceramide and sialyl-globotetraosylceramide, and the sulfated glycosphingolipids sulfatide, sulf-lactosylceramide, and sulf-globopentaosylceramide. A high cell surface expression of sialyl-lactotetra on hESC and human induced pluripotent stem cells (hiPSC) was demonstrated by flow cytometry, immunohistochemistry, and electron microscopy, whereas sulfated glycosphingolipids were only found in intracellular compartments. Immunohistochemistry showed distinct cell surface anti-sialyl-lactotetra staining on all seven hESC lines and three hiPSC lines analyzed, whereas no staining of hESC-derived hepatocyte-like or cardiomyocyte-like cells was obtained. Upon differentiation of hiPSC into hepatocyte-like cells, the sialyl-lactotetra epitope was rapidly down-regulated and not detectable after 14 days. These findings identify sialyl-lactotetra as a promising marker of undifferentiated human pluripotent stem cells.
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spelling pubmed-40819262014-07-18 Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells Barone, Angela Säljö, Karin Benktander, John Blomqvist, Maria Månsson, Jan-Eric Johansson, Bengt R. Mölne, Johan Aspegren, Anders Björquist, Petter Breimer, Michael E. Teneberg, Susann J Biol Chem Glycobiology and Extracellular Matrices Cell surface glycoconjugates are used as markers for undifferentiated pluripotent stem cells. Here, antibody binding and mass spectrometry characterization of acid glycosphingolipids isolated from a large number (1 × 10(9) cells) of human embryonic stem cell (hESC) lines allowed identification of several novel acid glycosphingolipids, like the gangliosides sialyl-lactotetraosylceramide and sialyl-globotetraosylceramide, and the sulfated glycosphingolipids sulfatide, sulf-lactosylceramide, and sulf-globopentaosylceramide. A high cell surface expression of sialyl-lactotetra on hESC and human induced pluripotent stem cells (hiPSC) was demonstrated by flow cytometry, immunohistochemistry, and electron microscopy, whereas sulfated glycosphingolipids were only found in intracellular compartments. Immunohistochemistry showed distinct cell surface anti-sialyl-lactotetra staining on all seven hESC lines and three hiPSC lines analyzed, whereas no staining of hESC-derived hepatocyte-like or cardiomyocyte-like cells was obtained. Upon differentiation of hiPSC into hepatocyte-like cells, the sialyl-lactotetra epitope was rapidly down-regulated and not detectable after 14 days. These findings identify sialyl-lactotetra as a promising marker of undifferentiated human pluripotent stem cells. American Society for Biochemistry and Molecular Biology 2014-07-04 2014-05-19 /pmc/articles/PMC4081926/ /pubmed/24841197 http://dx.doi.org/10.1074/jbc.M114.568832 Text en © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/) applies to Author Choice Articles
spellingShingle Glycobiology and Extracellular Matrices
Barone, Angela
Säljö, Karin
Benktander, John
Blomqvist, Maria
Månsson, Jan-Eric
Johansson, Bengt R.
Mölne, Johan
Aspegren, Anders
Björquist, Petter
Breimer, Michael E.
Teneberg, Susann
Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells
title Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells
title_full Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells
title_fullStr Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells
title_full_unstemmed Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells
title_short Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells
title_sort sialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells
topic Glycobiology and Extracellular Matrices
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081926/
https://www.ncbi.nlm.nih.gov/pubmed/24841197
http://dx.doi.org/10.1074/jbc.M114.568832
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