Cargando…
Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps)
The primary infection by Pneumocystis of normal, healthy infants is asymptomatic and goes undiagnosed. Microscopy diagnosis of Pneumocystis was sought in lung impression smears (LIS) from two ~3-month-old infants dying unexpectedly in the community. Pneumocystis nuclei and cysts were identified usin...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081978/ https://www.ncbi.nlm.nih.gov/pubmed/25003025 http://dx.doi.org/10.1016/j.mmcr.2014.06.001 |
_version_ | 1782324180752007168 |
---|---|
author | Ponce, Carolina A. Bustamante, Rebeca I. Gallo, Myriam Vargas, Sergio L. |
author_facet | Ponce, Carolina A. Bustamante, Rebeca I. Gallo, Myriam Vargas, Sergio L. |
author_sort | Ponce, Carolina A. |
collection | PubMed |
description | The primary infection by Pneumocystis of normal, healthy infants is asymptomatic and goes undiagnosed. Microscopy diagnosis of Pneumocystis was sought in lung impression smears (LIS) from two ~3-month-old infants dying unexpectedly in the community. Pneumocystis nuclei and cysts were identified using Hema-Gurr with subsequent Gomori–Grocott staining in the same spot documenting that these stains may be complementary. LIS provide for an observer–dependent, inexpensive, and ready-available method for detection of Pneumocystis in infant lungs. |
format | Online Article Text |
id | pubmed-4081978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-40819782014-07-07 Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps) Ponce, Carolina A. Bustamante, Rebeca I. Gallo, Myriam Vargas, Sergio L. Med Mycol Case Rep Article The primary infection by Pneumocystis of normal, healthy infants is asymptomatic and goes undiagnosed. Microscopy diagnosis of Pneumocystis was sought in lung impression smears (LIS) from two ~3-month-old infants dying unexpectedly in the community. Pneumocystis nuclei and cysts were identified using Hema-Gurr with subsequent Gomori–Grocott staining in the same spot documenting that these stains may be complementary. LIS provide for an observer–dependent, inexpensive, and ready-available method for detection of Pneumocystis in infant lungs. Elsevier 2014-06-20 /pmc/articles/PMC4081978/ /pubmed/25003025 http://dx.doi.org/10.1016/j.mmcr.2014.06.001 Text en © 2014 Published by Elsevier B.V. on behalf of International Society for Human and Animal Mycology. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Ponce, Carolina A. Bustamante, Rebeca I. Gallo, Myriam Vargas, Sergio L. Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps) |
title | Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps) |
title_full | Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps) |
title_fullStr | Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps) |
title_full_unstemmed | Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps) |
title_short | Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps) |
title_sort | diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081978/ https://www.ncbi.nlm.nih.gov/pubmed/25003025 http://dx.doi.org/10.1016/j.mmcr.2014.06.001 |
work_keys_str_mv | AT poncecarolinaa diagnosisoftheprimaryinfectionbypneumocystisinautopsyspecimensfromtwoinfantsusinglungimpressionsmearstouchpreps AT bustamanterebecai diagnosisoftheprimaryinfectionbypneumocystisinautopsyspecimensfromtwoinfantsusinglungimpressionsmearstouchpreps AT gallomyriam diagnosisoftheprimaryinfectionbypneumocystisinautopsyspecimensfromtwoinfantsusinglungimpressionsmearstouchpreps AT vargassergiol diagnosisoftheprimaryinfectionbypneumocystisinautopsyspecimensfromtwoinfantsusinglungimpressionsmearstouchpreps |