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Early Diagnosis of Leptospirosis

A 25-years old man from Zagreb, Croatia, was admitted to the University Hospital for Infectious Diseases four days after the onset of symptoms such as fever, intense pain in the calves and anuria. The patient owned a rabbit and, before the onset of the disease, repaired some rubber pipes damaged by...

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Autores principales: Babic-Erceg, Andrea, Karlovic-Martinkovic, Diana, Santini, Marija, Persic, Zdenka, Vilibic-Cavlek, Tatjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083297/
https://www.ncbi.nlm.nih.gov/pubmed/25002958
http://dx.doi.org/10.4081/idr.2014.5156
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author Babic-Erceg, Andrea
Karlovic-Martinkovic, Diana
Santini, Marija
Persic, Zdenka
Vilibic-Cavlek, Tatjana
author_facet Babic-Erceg, Andrea
Karlovic-Martinkovic, Diana
Santini, Marija
Persic, Zdenka
Vilibic-Cavlek, Tatjana
author_sort Babic-Erceg, Andrea
collection PubMed
description A 25-years old man from Zagreb, Croatia, was admitted to the University Hospital for Infectious Diseases four days after the onset of symptoms such as fever, intense pain in the calves and anuria. The patient owned a rabbit and, before the onset of the disease, repaired some rubber pipes damaged by rodents. At admission, he had a severe clinical picture with fever, hypotension, jaundice, immobility, and pain in leg muscles. Treatment with ceftriaxone was initiated in combination with volume restitution. Renal failure soon ensued. Consequently continuous venovenous hemodiaphiltration therapy was performed. Due to acute respiratory distress syndrome, the patient was mechanically ventilated. The patient’s condition gradually improved and he recovered fully from multi-organ failure. Diagnosis was confirmed by a microscopic agglutination test (MAT) covering 15 leptospira serovars and real-time polymerase-chain reaction (PCR). The first serum sample taken on day 6 tested negative for leptospira, while PCR showed positive results for leptospiral DNA. The second serum sample taken on day 13 tested positive for serovar Canicola serogroup Canicola, serovar Patoc, serovar Grippotyphosa serogroup Grippotyphosa and serovar Tarassovi serogroup Tarassovi (titre 4000, 4000, 1000 and 2000, respectively), while PCR was negative. This report highlights the benefits of combining MAT and PCR methods in early diagnosis of leptospirosis.
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spelling pubmed-40832972014-07-07 Early Diagnosis of Leptospirosis Babic-Erceg, Andrea Karlovic-Martinkovic, Diana Santini, Marija Persic, Zdenka Vilibic-Cavlek, Tatjana Infect Dis Rep Case Report A 25-years old man from Zagreb, Croatia, was admitted to the University Hospital for Infectious Diseases four days after the onset of symptoms such as fever, intense pain in the calves and anuria. The patient owned a rabbit and, before the onset of the disease, repaired some rubber pipes damaged by rodents. At admission, he had a severe clinical picture with fever, hypotension, jaundice, immobility, and pain in leg muscles. Treatment with ceftriaxone was initiated in combination with volume restitution. Renal failure soon ensued. Consequently continuous venovenous hemodiaphiltration therapy was performed. Due to acute respiratory distress syndrome, the patient was mechanically ventilated. The patient’s condition gradually improved and he recovered fully from multi-organ failure. Diagnosis was confirmed by a microscopic agglutination test (MAT) covering 15 leptospira serovars and real-time polymerase-chain reaction (PCR). The first serum sample taken on day 6 tested negative for leptospira, while PCR showed positive results for leptospiral DNA. The second serum sample taken on day 13 tested positive for serovar Canicola serogroup Canicola, serovar Patoc, serovar Grippotyphosa serogroup Grippotyphosa and serovar Tarassovi serogroup Tarassovi (titre 4000, 4000, 1000 and 2000, respectively), while PCR was negative. This report highlights the benefits of combining MAT and PCR methods in early diagnosis of leptospirosis. PAGEPress Publications, Pavia, Italy 2014-06-24 /pmc/articles/PMC4083297/ /pubmed/25002958 http://dx.doi.org/10.4081/idr.2014.5156 Text en ©Copyright A. Babic-Erceg et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Report
Babic-Erceg, Andrea
Karlovic-Martinkovic, Diana
Santini, Marija
Persic, Zdenka
Vilibic-Cavlek, Tatjana
Early Diagnosis of Leptospirosis
title Early Diagnosis of Leptospirosis
title_full Early Diagnosis of Leptospirosis
title_fullStr Early Diagnosis of Leptospirosis
title_full_unstemmed Early Diagnosis of Leptospirosis
title_short Early Diagnosis of Leptospirosis
title_sort early diagnosis of leptospirosis
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083297/
https://www.ncbi.nlm.nih.gov/pubmed/25002958
http://dx.doi.org/10.4081/idr.2014.5156
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