Cargando…

JNK3 Is Required for the Cytoprotective Effect of Exendin 4

Preservation of beta cell against apoptosis is one of the therapeutic benefits of the glucagon-like peptide-1 (GLP1) antidiabetic mimetics for preserving the functional beta cell mass exposed to diabetogenic condition including proinflammatory cytokines. The mitogen activated protein kinase 10 also...

Descripción completa

Detalles Bibliográficos
Autores principales: Ezanno, Hélène, Pawlowski, Valérie, Abdelli, Saida, Boutry, Raphael, Gmyr, Valery, Kerr-Conte, Julie, Bonny, Christophe, Pattou, François, Abderrahmani, Amar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083605/
https://www.ncbi.nlm.nih.gov/pubmed/25025079
http://dx.doi.org/10.1155/2014/814854
_version_ 1782324401175265280
author Ezanno, Hélène
Pawlowski, Valérie
Abdelli, Saida
Boutry, Raphael
Gmyr, Valery
Kerr-Conte, Julie
Bonny, Christophe
Pattou, François
Abderrahmani, Amar
author_facet Ezanno, Hélène
Pawlowski, Valérie
Abdelli, Saida
Boutry, Raphael
Gmyr, Valery
Kerr-Conte, Julie
Bonny, Christophe
Pattou, François
Abderrahmani, Amar
author_sort Ezanno, Hélène
collection PubMed
description Preservation of beta cell against apoptosis is one of the therapeutic benefits of the glucagon-like peptide-1 (GLP1) antidiabetic mimetics for preserving the functional beta cell mass exposed to diabetogenic condition including proinflammatory cytokines. The mitogen activated protein kinase 10 also called c-jun amino-terminal kinase 3 (JNK3) plays a protective role in insulin-secreting cells against death caused by cytokines. In this study, we investigated whether the JNK3 expression is associated with the protective effect elicited by the GLP1 mimetic exendin 4. We found an increase in the abundance of JNK3 in isolated human islets and INS-1E cells cultured with exendin 4. Induction of JNK3 by exendin 4 was associated with an increased survival of INS-1E cells. Silencing of JNK3 prevented the cytoprotective effect of exendin 4 against apoptosis elicited by culture condition and cytokines. These results emphasize the requirement of JNK3 in the antiapoptotic effects of exendin 4.
format Online
Article
Text
id pubmed-4083605
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-40836052014-07-14 JNK3 Is Required for the Cytoprotective Effect of Exendin 4 Ezanno, Hélène Pawlowski, Valérie Abdelli, Saida Boutry, Raphael Gmyr, Valery Kerr-Conte, Julie Bonny, Christophe Pattou, François Abderrahmani, Amar J Diabetes Res Research Article Preservation of beta cell against apoptosis is one of the therapeutic benefits of the glucagon-like peptide-1 (GLP1) antidiabetic mimetics for preserving the functional beta cell mass exposed to diabetogenic condition including proinflammatory cytokines. The mitogen activated protein kinase 10 also called c-jun amino-terminal kinase 3 (JNK3) plays a protective role in insulin-secreting cells against death caused by cytokines. In this study, we investigated whether the JNK3 expression is associated with the protective effect elicited by the GLP1 mimetic exendin 4. We found an increase in the abundance of JNK3 in isolated human islets and INS-1E cells cultured with exendin 4. Induction of JNK3 by exendin 4 was associated with an increased survival of INS-1E cells. Silencing of JNK3 prevented the cytoprotective effect of exendin 4 against apoptosis elicited by culture condition and cytokines. These results emphasize the requirement of JNK3 in the antiapoptotic effects of exendin 4. Hindawi Publishing Corporation 2014 2014-06-16 /pmc/articles/PMC4083605/ /pubmed/25025079 http://dx.doi.org/10.1155/2014/814854 Text en Copyright © 2014 Hélène Ezanno et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ezanno, Hélène
Pawlowski, Valérie
Abdelli, Saida
Boutry, Raphael
Gmyr, Valery
Kerr-Conte, Julie
Bonny, Christophe
Pattou, François
Abderrahmani, Amar
JNK3 Is Required for the Cytoprotective Effect of Exendin 4
title JNK3 Is Required for the Cytoprotective Effect of Exendin 4
title_full JNK3 Is Required for the Cytoprotective Effect of Exendin 4
title_fullStr JNK3 Is Required for the Cytoprotective Effect of Exendin 4
title_full_unstemmed JNK3 Is Required for the Cytoprotective Effect of Exendin 4
title_short JNK3 Is Required for the Cytoprotective Effect of Exendin 4
title_sort jnk3 is required for the cytoprotective effect of exendin 4
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083605/
https://www.ncbi.nlm.nih.gov/pubmed/25025079
http://dx.doi.org/10.1155/2014/814854
work_keys_str_mv AT ezannohelene jnk3isrequiredforthecytoprotectiveeffectofexendin4
AT pawlowskivalerie jnk3isrequiredforthecytoprotectiveeffectofexendin4
AT abdellisaida jnk3isrequiredforthecytoprotectiveeffectofexendin4
AT boutryraphael jnk3isrequiredforthecytoprotectiveeffectofexendin4
AT gmyrvalery jnk3isrequiredforthecytoprotectiveeffectofexendin4
AT kerrcontejulie jnk3isrequiredforthecytoprotectiveeffectofexendin4
AT bonnychristophe jnk3isrequiredforthecytoprotectiveeffectofexendin4
AT pattoufrancois jnk3isrequiredforthecytoprotectiveeffectofexendin4
AT abderrahmaniamar jnk3isrequiredforthecytoprotectiveeffectofexendin4