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Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil

OBJECTIVE: To compare the accuracy of the amplified Mycobacterium tuberculosis direct (AMTD) test with reference methods for the laboratory diagnosis of tuberculosis in HIV-infected patients. METHODS: This was a study of diagnostic accuracy comparing AMTD test results with those obtained by culture...

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Autores principales: Barreto, Leonardo Bruno Paz Ferreira, Lourenço, Maria Cristina da Silva, Rolla, Valéria Cavalcanti, Veloso, Valdiléia Gonçalves, Huf, Gisele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Pneumologia e Tisiologia 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083646/
https://www.ncbi.nlm.nih.gov/pubmed/24831399
http://dx.doi.org/10.1590/S1806-37132014000200008
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author Barreto, Leonardo Bruno Paz Ferreira
Lourenço, Maria Cristina da Silva
Rolla, Valéria Cavalcanti
Veloso, Valdiléia Gonçalves
Huf, Gisele
author_facet Barreto, Leonardo Bruno Paz Ferreira
Lourenço, Maria Cristina da Silva
Rolla, Valéria Cavalcanti
Veloso, Valdiléia Gonçalves
Huf, Gisele
author_sort Barreto, Leonardo Bruno Paz Ferreira
collection PubMed
description OBJECTIVE: To compare the accuracy of the amplified Mycobacterium tuberculosis direct (AMTD) test with reference methods for the laboratory diagnosis of tuberculosis in HIV-infected patients. METHODS: This was a study of diagnostic accuracy comparing AMTD test results with those obtained by culture on Löwenstein-Jensen (LJ) medium and by the BACTEC Mycobacteria Growth Indicator Tube 960 (BACTEC MGIT 960) system in respiratory samples analyzed at the Bioassay and Bacteriology Laboratory of the Oswaldo Cruz Foundation Evandro Chagas Clinical Research Institute in the city of Rio de Janeiro, Brazil. RESULTS: We analyzed respiratory samples collected from 118 patients, of whom 88 (74.4%) were male. The mean age was 36.6 ± 10.6 years. Using the AMTD test, the BACTEC MGIT 960 system, and LJ culture, we identified M. tuberculosis complex in 31.0%, 29.7%, and 27.1% of the samples, respectively. In comparison with LJ culture, the AMTD test had a sensitivity, specificity, positive predictive value, and negative predictive value of 87.5%, 89.4%, 75.7%, and 95.0%, respectively, for LJ culture, whereas, in comparison with the BACTEC MGIT 960 system, it showed values of 88.6%, 92.4%, 83.8%, and 94.8%, respectively. CONCLUSIONS: The AMTD test showed good sensitivity and specificity in the population studied, enabling the laboratory detection of M. tuberculosis complex in paucibacillary respiratory specimens.
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spelling pubmed-40836462014-07-16 Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil Barreto, Leonardo Bruno Paz Ferreira Lourenço, Maria Cristina da Silva Rolla, Valéria Cavalcanti Veloso, Valdiléia Gonçalves Huf, Gisele J Bras Pneumol Original Articles OBJECTIVE: To compare the accuracy of the amplified Mycobacterium tuberculosis direct (AMTD) test with reference methods for the laboratory diagnosis of tuberculosis in HIV-infected patients. METHODS: This was a study of diagnostic accuracy comparing AMTD test results with those obtained by culture on Löwenstein-Jensen (LJ) medium and by the BACTEC Mycobacteria Growth Indicator Tube 960 (BACTEC MGIT 960) system in respiratory samples analyzed at the Bioassay and Bacteriology Laboratory of the Oswaldo Cruz Foundation Evandro Chagas Clinical Research Institute in the city of Rio de Janeiro, Brazil. RESULTS: We analyzed respiratory samples collected from 118 patients, of whom 88 (74.4%) were male. The mean age was 36.6 ± 10.6 years. Using the AMTD test, the BACTEC MGIT 960 system, and LJ culture, we identified M. tuberculosis complex in 31.0%, 29.7%, and 27.1% of the samples, respectively. In comparison with LJ culture, the AMTD test had a sensitivity, specificity, positive predictive value, and negative predictive value of 87.5%, 89.4%, 75.7%, and 95.0%, respectively, for LJ culture, whereas, in comparison with the BACTEC MGIT 960 system, it showed values of 88.6%, 92.4%, 83.8%, and 94.8%, respectively. CONCLUSIONS: The AMTD test showed good sensitivity and specificity in the population studied, enabling the laboratory detection of M. tuberculosis complex in paucibacillary respiratory specimens. Sociedade Brasileira de Pneumologia e Tisiologia 2014 /pmc/articles/PMC4083646/ /pubmed/24831399 http://dx.doi.org/10.1590/S1806-37132014000200008 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Barreto, Leonardo Bruno Paz Ferreira
Lourenço, Maria Cristina da Silva
Rolla, Valéria Cavalcanti
Veloso, Valdiléia Gonçalves
Huf, Gisele
Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil
title Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil
title_full Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil
title_fullStr Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil
title_full_unstemmed Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil
title_short Use of amplified Mycobacterium tuberculosis direct test in respiratory samples from HIV-infected patients in Brazil
title_sort use of amplified mycobacterium tuberculosis direct test in respiratory samples from hiv-infected patients in brazil
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083646/
https://www.ncbi.nlm.nih.gov/pubmed/24831399
http://dx.doi.org/10.1590/S1806-37132014000200008
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