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Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents

Dioctadecyldimethylammonium bromide (DODAB) is an antimicrobial lipid that can be dispersed as large closed bilayers (LV) or bilayer disks (BF). Gramicidin (Gr) is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations. In mammalian cells, DODA...

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Autores principales: Ragioto, Danielle AMT, Carrasco, Letícia DM, Carmona-Ribeiro, Ana M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085336/
https://www.ncbi.nlm.nih.gov/pubmed/25061295
http://dx.doi.org/10.2147/IJN.S65289
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author Ragioto, Danielle AMT
Carrasco, Letícia DM
Carmona-Ribeiro, Ana M
author_facet Ragioto, Danielle AMT
Carrasco, Letícia DM
Carmona-Ribeiro, Ana M
author_sort Ragioto, Danielle AMT
collection PubMed
description Dioctadecyldimethylammonium bromide (DODAB) is an antimicrobial lipid that can be dispersed as large closed bilayers (LV) or bilayer disks (BF). Gramicidin (Gr) is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations. In mammalian cells, DODAB and Gr have the drawbacks of Gram-positive resistance and high toxicity, respectively. In this study, DODAB bilayers incorporating Gr showed good antimicrobial activity and low toxicity. Techniques employed were spectroscopy, photon correlation spectroscopy for sizing and evaluation of the surface potential at the shear plane, turbidimetric detection of dissipation of osmotic gradients in LV/Gr, determination of bacterial cell lysis, and counting of colony-forming units. There was quantitative incorporation of Gr and development of functional channels in LV. Gr increased the bilayer charge density in LV but did not affect the BF charge density, with localization of Gr at the BF borders. DODAB/Gr formulations substantially reduce Gr toxicity against eukaryotic cells and advantageously broaden the antimicrobial activity spectrum, effectively killing Escherichia coli and Staphylococcus aureus bacteria with occurrence of cell lysis.
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spelling pubmed-40853362014-07-24 Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents Ragioto, Danielle AMT Carrasco, Letícia DM Carmona-Ribeiro, Ana M Int J Nanomedicine Original Research Dioctadecyldimethylammonium bromide (DODAB) is an antimicrobial lipid that can be dispersed as large closed bilayers (LV) or bilayer disks (BF). Gramicidin (Gr) is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations. In mammalian cells, DODAB and Gr have the drawbacks of Gram-positive resistance and high toxicity, respectively. In this study, DODAB bilayers incorporating Gr showed good antimicrobial activity and low toxicity. Techniques employed were spectroscopy, photon correlation spectroscopy for sizing and evaluation of the surface potential at the shear plane, turbidimetric detection of dissipation of osmotic gradients in LV/Gr, determination of bacterial cell lysis, and counting of colony-forming units. There was quantitative incorporation of Gr and development of functional channels in LV. Gr increased the bilayer charge density in LV but did not affect the BF charge density, with localization of Gr at the BF borders. DODAB/Gr formulations substantially reduce Gr toxicity against eukaryotic cells and advantageously broaden the antimicrobial activity spectrum, effectively killing Escherichia coli and Staphylococcus aureus bacteria with occurrence of cell lysis. Dove Medical Press 2014-06-30 /pmc/articles/PMC4085336/ /pubmed/25061295 http://dx.doi.org/10.2147/IJN.S65289 Text en © 2014 Ragioto et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Ragioto, Danielle AMT
Carrasco, Letícia DM
Carmona-Ribeiro, Ana M
Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_full Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_fullStr Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_full_unstemmed Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_short Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_sort novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085336/
https://www.ncbi.nlm.nih.gov/pubmed/25061295
http://dx.doi.org/10.2147/IJN.S65289
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