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C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization

Cytokinesis generally produces two separate daughter cells, but in some tissues daughter nuclei remain connected to a shared cytoplasm, or syncytium, through incomplete cytokinesis. How syncytia form remains poorly understood. We studied syncytial formation in the Caenorhabditis elegans germline, in...

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Autores principales: Amini, Rana, Goupil, Eugénie, Labella, Sara, Zetka, Monique, Maddox, Amy S., Labbé, Jean-Claude, Chartier, Nicolas T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085705/
https://www.ncbi.nlm.nih.gov/pubmed/24982432
http://dx.doi.org/10.1083/jcb.201310117
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author Amini, Rana
Goupil, Eugénie
Labella, Sara
Zetka, Monique
Maddox, Amy S.
Labbé, Jean-Claude
Chartier, Nicolas T.
author_facet Amini, Rana
Goupil, Eugénie
Labella, Sara
Zetka, Monique
Maddox, Amy S.
Labbé, Jean-Claude
Chartier, Nicolas T.
author_sort Amini, Rana
collection PubMed
description Cytokinesis generally produces two separate daughter cells, but in some tissues daughter nuclei remain connected to a shared cytoplasm, or syncytium, through incomplete cytokinesis. How syncytia form remains poorly understood. We studied syncytial formation in the Caenorhabditis elegans germline, in which germ cells connect to a shared cytoplasm core (the rachis) via intercellular bridges. We found that syncytial architecture initiates early in larval development, and germ cells become progressively interconnected until adulthood. The short Anillin family scaffold protein ANI-2 is enriched at intercellular bridges from the onset of germ cell specification, and ANI-2 loss resulted in destabilization of intercellular bridges and germ cell multinucleation defects. These defects were partially rescued by depleting the canonical Anillin ANI-1 or blocking cytoplasmic streaming. ANI-2 is also required for elastic deformation of the gonad during ovulation. We propose that ANI-2 promotes germ cell syncytial organization and allows for compensation of the mechanical stress associated with oogenesis by conferring stability and elasticity to germ cell intercellular bridges.
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spelling pubmed-40857052015-01-07 C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization Amini, Rana Goupil, Eugénie Labella, Sara Zetka, Monique Maddox, Amy S. Labbé, Jean-Claude Chartier, Nicolas T. J Cell Biol Research Articles Cytokinesis generally produces two separate daughter cells, but in some tissues daughter nuclei remain connected to a shared cytoplasm, or syncytium, through incomplete cytokinesis. How syncytia form remains poorly understood. We studied syncytial formation in the Caenorhabditis elegans germline, in which germ cells connect to a shared cytoplasm core (the rachis) via intercellular bridges. We found that syncytial architecture initiates early in larval development, and germ cells become progressively interconnected until adulthood. The short Anillin family scaffold protein ANI-2 is enriched at intercellular bridges from the onset of germ cell specification, and ANI-2 loss resulted in destabilization of intercellular bridges and germ cell multinucleation defects. These defects were partially rescued by depleting the canonical Anillin ANI-1 or blocking cytoplasmic streaming. ANI-2 is also required for elastic deformation of the gonad during ovulation. We propose that ANI-2 promotes germ cell syncytial organization and allows for compensation of the mechanical stress associated with oogenesis by conferring stability and elasticity to germ cell intercellular bridges. The Rockefeller University Press 2014-07-07 /pmc/articles/PMC4085705/ /pubmed/24982432 http://dx.doi.org/10.1083/jcb.201310117 Text en © 2014 Amini et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Amini, Rana
Goupil, Eugénie
Labella, Sara
Zetka, Monique
Maddox, Amy S.
Labbé, Jean-Claude
Chartier, Nicolas T.
C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization
title C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization
title_full C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization
title_fullStr C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization
title_full_unstemmed C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization
title_short C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization
title_sort c. elegans anillin proteins regulate intercellular bridge stability and germline syncytial organization
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085705/
https://www.ncbi.nlm.nih.gov/pubmed/24982432
http://dx.doi.org/10.1083/jcb.201310117
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