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Bud3 activates Cdc42 to establish a proper growth site in budding yeast
Cell polarization occurs along a single axis that is generally determined by a spatial cue, yet the underlying mechanism is poorly understood. Using biochemical assays and live-cell imaging, we show that cell polarization to a proper growth site requires activation of Cdc42 by Bud3 in haploid buddin...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085707/ https://www.ncbi.nlm.nih.gov/pubmed/25002677 http://dx.doi.org/10.1083/jcb.201402040 |
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author | Kang, Pil Jung Lee, Mid Eum Park, Hay-Oak |
author_facet | Kang, Pil Jung Lee, Mid Eum Park, Hay-Oak |
author_sort | Kang, Pil Jung |
collection | PubMed |
description | Cell polarization occurs along a single axis that is generally determined by a spatial cue, yet the underlying mechanism is poorly understood. Using biochemical assays and live-cell imaging, we show that cell polarization to a proper growth site requires activation of Cdc42 by Bud3 in haploid budding yeast. Bud3 catalyzes the release of guanosine diphosphate (GDP) from Cdc42 and elevates intracellular Cdc42–guanosine triphosphate (GTP) levels in cells with inactive Cdc24, which has as of yet been the sole GDP–GTP exchange factor for Cdc42. Cdc42 is activated in two temporal steps in the G1 phase: the first depends on Bud3, whereas subsequent activation depends on Cdc24. Mutational analyses suggest that biphasic activation of Cdc42 in G1 is necessary for assembly of a proper bud site. Biphasic activation of Cdc42 or Rac GTPases may be a general mechanism for spatial cue–directed cell polarization in eukaryotes. |
format | Online Article Text |
id | pubmed-4085707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-40857072015-01-07 Bud3 activates Cdc42 to establish a proper growth site in budding yeast Kang, Pil Jung Lee, Mid Eum Park, Hay-Oak J Cell Biol Research Articles Cell polarization occurs along a single axis that is generally determined by a spatial cue, yet the underlying mechanism is poorly understood. Using biochemical assays and live-cell imaging, we show that cell polarization to a proper growth site requires activation of Cdc42 by Bud3 in haploid budding yeast. Bud3 catalyzes the release of guanosine diphosphate (GDP) from Cdc42 and elevates intracellular Cdc42–guanosine triphosphate (GTP) levels in cells with inactive Cdc24, which has as of yet been the sole GDP–GTP exchange factor for Cdc42. Cdc42 is activated in two temporal steps in the G1 phase: the first depends on Bud3, whereas subsequent activation depends on Cdc24. Mutational analyses suggest that biphasic activation of Cdc42 in G1 is necessary for assembly of a proper bud site. Biphasic activation of Cdc42 or Rac GTPases may be a general mechanism for spatial cue–directed cell polarization in eukaryotes. The Rockefeller University Press 2014-07-07 /pmc/articles/PMC4085707/ /pubmed/25002677 http://dx.doi.org/10.1083/jcb.201402040 Text en © 2014 Kang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Kang, Pil Jung Lee, Mid Eum Park, Hay-Oak Bud3 activates Cdc42 to establish a proper growth site in budding yeast |
title | Bud3 activates Cdc42 to establish a proper growth site in budding yeast |
title_full | Bud3 activates Cdc42 to establish a proper growth site in budding yeast |
title_fullStr | Bud3 activates Cdc42 to establish a proper growth site in budding yeast |
title_full_unstemmed | Bud3 activates Cdc42 to establish a proper growth site in budding yeast |
title_short | Bud3 activates Cdc42 to establish a proper growth site in budding yeast |
title_sort | bud3 activates cdc42 to establish a proper growth site in budding yeast |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085707/ https://www.ncbi.nlm.nih.gov/pubmed/25002677 http://dx.doi.org/10.1083/jcb.201402040 |
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