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CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)

The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY anti...

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Autores principales: Aguilar, Irma, Sánchez, Elda E., Girón, María E., Estrella, Amalid, Guerrero, Belsy, Rodriguez-Acosta, F. Alexis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085830/
https://www.ncbi.nlm.nih.gov/pubmed/24553610
http://dx.doi.org/10.1590/S0036-46652014000100009
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author Aguilar, Irma
Sánchez, Elda E.
Girón, María E.
Estrella, Amalid
Guerrero, Belsy
Rodriguez-Acosta, F. Alexis
author_facet Aguilar, Irma
Sánchez, Elda E.
Girón, María E.
Estrella, Amalid
Guerrero, Belsy
Rodriguez-Acosta, F. Alexis
author_sort Aguilar, Irma
collection PubMed
description The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY antivenom consisting of polyclonal antibodies against coral snake venom in the egg yolk of immunized hens. We have adapted a reported process of antibody purification from egg yolks, and achieved 90% antibody purity. The customized technique consisted of the removal of lipids from distilled water-diluted egg yolks by a freeze–thaw sequence. The specific immunoglobulins were present in the egg yolk for up to 180 days postimmunization. Therefore, by means of small venom quantities, a significant amount of immunoglobulins were found in an adequately purified state (The obtained material contained about 90% pure IgY). The antigen binding of the immunoglobulins was detected by a double immunodiffusion test. Titers of antibodies in the yolk were estimated with a serum protection assay (Median effective dose = ED(50)) (ED(50)= 477 mg/kg). Given that breeding hens is economically feasible, egg gathering is noninvasive and the purification of IgY antibodies is quick and easy, chicken immunization is an excellent alternative for the production of polyclonal antibodies. To the best of our knowledge, this is the first coral snake antivenom prepared in birds.
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spelling pubmed-40858302014-07-16 CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus) Aguilar, Irma Sánchez, Elda E. Girón, María E. Estrella, Amalid Guerrero, Belsy Rodriguez-Acosta, F. Alexis Rev Inst Med Trop Sao Paulo Animal Envenomation The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY antivenom consisting of polyclonal antibodies against coral snake venom in the egg yolk of immunized hens. We have adapted a reported process of antibody purification from egg yolks, and achieved 90% antibody purity. The customized technique consisted of the removal of lipids from distilled water-diluted egg yolks by a freeze–thaw sequence. The specific immunoglobulins were present in the egg yolk for up to 180 days postimmunization. Therefore, by means of small venom quantities, a significant amount of immunoglobulins were found in an adequately purified state (The obtained material contained about 90% pure IgY). The antigen binding of the immunoglobulins was detected by a double immunodiffusion test. Titers of antibodies in the yolk were estimated with a serum protection assay (Median effective dose = ED(50)) (ED(50)= 477 mg/kg). Given that breeding hens is economically feasible, egg gathering is noninvasive and the purification of IgY antibodies is quick and easy, chicken immunization is an excellent alternative for the production of polyclonal antibodies. To the best of our knowledge, this is the first coral snake antivenom prepared in birds. Instituto de Medicina Tropical 2014 /pmc/articles/PMC4085830/ /pubmed/24553610 http://dx.doi.org/10.1590/S0036-46652014000100009 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Animal Envenomation
Aguilar, Irma
Sánchez, Elda E.
Girón, María E.
Estrella, Amalid
Guerrero, Belsy
Rodriguez-Acosta, F. Alexis
CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
title CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
title_full CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
title_fullStr CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
title_full_unstemmed CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
title_short CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
title_sort coral snake antivenom produced in chickens (gallus domesticus)
topic Animal Envenomation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085830/
https://www.ncbi.nlm.nih.gov/pubmed/24553610
http://dx.doi.org/10.1590/S0036-46652014000100009
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