MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS

A novel SYBR(®) green-real time polymerase chain reaction (qPCR) was developed to detect two Bartonella species, B. henselae and B. clarridgeiae, directly from blood samples. The test was used in blood samples obtained from cats living in animal shelters in Southern Brazil. Results were compared wit...

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Autores principales: Staggemeier, Rodrigo, Pilger, Diogo André, Spilki, Fernando Rosado, Cantarelli, Vlademir Vicente
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2014
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085843/
https://www.ncbi.nlm.nih.gov/pubmed/24626408
http://dx.doi.org/10.1590/S0036-46652014000200001
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author Staggemeier, Rodrigo
Pilger, Diogo André
Spilki, Fernando Rosado
Cantarelli, Vlademir Vicente
author_facet Staggemeier, Rodrigo
Pilger, Diogo André
Spilki, Fernando Rosado
Cantarelli, Vlademir Vicente
author_sort Staggemeier, Rodrigo
collection PubMed
description A novel SYBR(®) green-real time polymerase chain reaction (qPCR) was developed to detect two Bartonella species, B. henselae and B. clarridgeiae, directly from blood samples. The test was used in blood samples obtained from cats living in animal shelters in Southern Brazil. Results were compared with those obtained by conventional PCR targeting Bartonella spp. Among the 47 samples analyzed, eight were positive using the conventional PCR and 12 were positive using qPCR. Importantly, the new qPCR detected the presence of both B. henselae and B. clarridgeiae in two samples. The results show that the qPCR described here may be a reliable tool for the screening and differentiation of two important Bartonella species.
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spelling pubmed-40858432014-07-16 MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS Staggemeier, Rodrigo Pilger, Diogo André Spilki, Fernando Rosado Cantarelli, Vlademir Vicente Rev Inst Med Trop Sao Paulo Microbiology A novel SYBR(®) green-real time polymerase chain reaction (qPCR) was developed to detect two Bartonella species, B. henselae and B. clarridgeiae, directly from blood samples. The test was used in blood samples obtained from cats living in animal shelters in Southern Brazil. Results were compared with those obtained by conventional PCR targeting Bartonella spp. Among the 47 samples analyzed, eight were positive using the conventional PCR and 12 were positive using qPCR. Importantly, the new qPCR detected the presence of both B. henselae and B. clarridgeiae in two samples. The results show that the qPCR described here may be a reliable tool for the screening and differentiation of two important Bartonella species. Instituto de Medicina Tropical 2014 /pmc/articles/PMC4085843/ /pubmed/24626408 http://dx.doi.org/10.1590/S0036-46652014000200001 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Microbiology
Staggemeier, Rodrigo
Pilger, Diogo André
Spilki, Fernando Rosado
Cantarelli, Vlademir Vicente
MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS
title MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS
title_full MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS
title_fullStr MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS
title_full_unstemmed MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS
title_short MULTIPLEX SYBR(®) GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS
title_sort multiplex sybr(®) green-real time pcr (qpcr) assay for the detection and differentiation of bartonella henselae and bartonella clarridgeiae in cats
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085843/
https://www.ncbi.nlm.nih.gov/pubmed/24626408
http://dx.doi.org/10.1590/S0036-46652014000200001
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