Photosynthetic flexibility in maize exposed to salinity and shade

C(4) photosynthesis involves a close collaboration of the C(3) and C(4) metabolic cycles across the mesophyll and bundle-sheath cells. This study investigated the coordination of C(4) photosynthesis in maize plants subjected to two salinity (50 and 100mM NaCl) treatments and one shade (20% of full sunlight) treatment. Photosynthetic efficiency was probed by combining leaf gas-exchange measurements with carbon isotope discrimination and assaying the key carboxylases [ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC)] and decarboxylases [nicotinamide adenine dinucleotide phosphate malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PEP-CK)] operating in maize leaves. Generally, salinity inhibited plant growth and photosynthesis to a lesser extent than shade. Salinity reduced photosynthesis primarily by reducing stomatal conductance and secondarily by equally reducing Rubisco and PEPC activities; the decarboxylases were inhibited more than the carboxylases. Salinity increased photosynthetic carbon isotope discrimination (Δ(p)) and reduced leaf dry-matter carbon isotope composition ((13)δ) due to changes in p (i)/p (a) (intercellular to ambient CO(2) partial pressure), while CO(2) leakiness out of the bundle sheath (ϕ) was similar to that in control plants. Acclimation to shade was underpinned by a greater downregulation of PEPC relative to Rubisco activity, and a lesser inhibition of NADP-ME (primary decarboxylase) relative to PEP-CK (secondary decarboxylase). Shade reduced Δ(p) and ɸ without significantly affecting leaf (13)δ or p (i)/p (a) relative to control plants. Accordingly, shade perturbed the balance between the C(3) and C(4) cycles during photosynthesis in maize, and demonstrated the flexible partitioning of C(4) acid decarboxylation activity between NADP-ME and PEP-CK in response to the environment. This study highlights the need to improve our understanding of the links between leaf (13)δ and photosynthetic Δ(p), and the role of the secondary decarboxylase PEP-CK in NADP-ME plants such as maize.