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Exposure to Silica Nanoparticles Causes Reversible Damage of the Spermatogenic Process in Mice

Environmental exposure to nanomaterials is inevitable, as nanomaterials have become part of our daily life now. In this study, we firstly investigated the effects of silica nanoparticles on the spermatogenic process according to their time course in male mice. 48 male mice were randomly divided into...

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Autores principales: Xu, Ying, Wang, Na, Yu, Yang, Li, Yang, Li, Yan-Bo, Yu, Yong-Bo, Zhou, Xian-Qing, Sun, Zhi-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086902/
https://www.ncbi.nlm.nih.gov/pubmed/25003337
http://dx.doi.org/10.1371/journal.pone.0101572
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author Xu, Ying
Wang, Na
Yu, Yang
Li, Yang
Li, Yan-Bo
Yu, Yong-Bo
Zhou, Xian-Qing
Sun, Zhi-Wei
author_facet Xu, Ying
Wang, Na
Yu, Yang
Li, Yang
Li, Yan-Bo
Yu, Yong-Bo
Zhou, Xian-Qing
Sun, Zhi-Wei
author_sort Xu, Ying
collection PubMed
description Environmental exposure to nanomaterials is inevitable, as nanomaterials have become part of our daily life now. In this study, we firstly investigated the effects of silica nanoparticles on the spermatogenic process according to their time course in male mice. 48 male mice were randomly divided into control group and silica nanoparticle group with 24 mice per group, with three evaluation time points (15, 35 and 60 days after the first dose) per group. Mice were exposed to the vehicle control and silica nanoparticles at a dosage of 20 mg/kg every 3 days, five times over a 13-day period, and were sacrificed at 15, 35 and 60 days after the first dose. The results showed that silica nanoparticles caused damage to the mitochondrial cristae and decreased the levels of ATP, resulting in oxidative stress in the testis by days 15 and 35; however, the damage was repaired by day 60. DNA damage and the decreases in the quantity and quality of epididymal sperm were found by days 15 and 35; but these changes were recovered by day 60. In contrast, the acrosome integrity and fertility in epididymal sperm, the numbers of spermatogonia and sperm in the testes, and the levels of three major sex hormones were not significantly affected throughout the 60-day period. The results suggest that nanoparticles can cause reversible damage to the sperms in the epididymis without affecting fertility, they are more sensitive than both spermatogonia and spermatocytes to silica nanoparticle toxicity. Considering the spermatogenesis time course, silica nanoparticles primarily influence the maturation process of sperm in the epididymis by causing oxidative stress and damage to the mitochondrial structure, resulting in energy metabolism dysfunction.
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spelling pubmed-40869022014-07-14 Exposure to Silica Nanoparticles Causes Reversible Damage of the Spermatogenic Process in Mice Xu, Ying Wang, Na Yu, Yang Li, Yang Li, Yan-Bo Yu, Yong-Bo Zhou, Xian-Qing Sun, Zhi-Wei PLoS One Research Article Environmental exposure to nanomaterials is inevitable, as nanomaterials have become part of our daily life now. In this study, we firstly investigated the effects of silica nanoparticles on the spermatogenic process according to their time course in male mice. 48 male mice were randomly divided into control group and silica nanoparticle group with 24 mice per group, with three evaluation time points (15, 35 and 60 days after the first dose) per group. Mice were exposed to the vehicle control and silica nanoparticles at a dosage of 20 mg/kg every 3 days, five times over a 13-day period, and were sacrificed at 15, 35 and 60 days after the first dose. The results showed that silica nanoparticles caused damage to the mitochondrial cristae and decreased the levels of ATP, resulting in oxidative stress in the testis by days 15 and 35; however, the damage was repaired by day 60. DNA damage and the decreases in the quantity and quality of epididymal sperm were found by days 15 and 35; but these changes were recovered by day 60. In contrast, the acrosome integrity and fertility in epididymal sperm, the numbers of spermatogonia and sperm in the testes, and the levels of three major sex hormones were not significantly affected throughout the 60-day period. The results suggest that nanoparticles can cause reversible damage to the sperms in the epididymis without affecting fertility, they are more sensitive than both spermatogonia and spermatocytes to silica nanoparticle toxicity. Considering the spermatogenesis time course, silica nanoparticles primarily influence the maturation process of sperm in the epididymis by causing oxidative stress and damage to the mitochondrial structure, resulting in energy metabolism dysfunction. Public Library of Science 2014-07-08 /pmc/articles/PMC4086902/ /pubmed/25003337 http://dx.doi.org/10.1371/journal.pone.0101572 Text en © 2014 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xu, Ying
Wang, Na
Yu, Yang
Li, Yang
Li, Yan-Bo
Yu, Yong-Bo
Zhou, Xian-Qing
Sun, Zhi-Wei
Exposure to Silica Nanoparticles Causes Reversible Damage of the Spermatogenic Process in Mice
title Exposure to Silica Nanoparticles Causes Reversible Damage of the Spermatogenic Process in Mice
title_full Exposure to Silica Nanoparticles Causes Reversible Damage of the Spermatogenic Process in Mice
title_fullStr Exposure to Silica Nanoparticles Causes Reversible Damage of the Spermatogenic Process in Mice
title_full_unstemmed Exposure to Silica Nanoparticles Causes Reversible Damage of the Spermatogenic Process in Mice
title_short Exposure to Silica Nanoparticles Causes Reversible Damage of the Spermatogenic Process in Mice
title_sort exposure to silica nanoparticles causes reversible damage of the spermatogenic process in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086902/
https://www.ncbi.nlm.nih.gov/pubmed/25003337
http://dx.doi.org/10.1371/journal.pone.0101572
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