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Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells
In metastasis, circulating tumor cells penetrate the walls of blood vessels and enter the metastatic target tissue, thereby becoming exposed to novel and relatively unsupportive microenvironments. In the new microenvironments, the tumor cells often remain in a dormant state indefinitely and must ada...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086928/ https://www.ncbi.nlm.nih.gov/pubmed/25004123 http://dx.doi.org/10.1371/journal.pone.0101160 |
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author | Rondeau, Gaelle Abedinpour, Parisa Desai, Prerak Baron, Veronique T. Borgstrom, Per Welsh, John |
author_facet | Rondeau, Gaelle Abedinpour, Parisa Desai, Prerak Baron, Veronique T. Borgstrom, Per Welsh, John |
author_sort | Rondeau, Gaelle |
collection | PubMed |
description | In metastasis, circulating tumor cells penetrate the walls of blood vessels and enter the metastatic target tissue, thereby becoming exposed to novel and relatively unsupportive microenvironments. In the new microenvironments, the tumor cells often remain in a dormant state indefinitely and must adapt before they are able to successfully colonize the tissue. Very little is known about this adaptive process. We studied temporal changes in gene expression when breast cancer cells adapt to survive and grow on brain, bone marrow, and lung tissue maintained in an in vivo culture system, as models of the metastatic colonization of these tissues. We observed the transient activation of genes typically associated with homeostasis and stress during the initial stages of adaptation, followed by the activation of genes that mediate more advanced functions, such as elaboration of cell morphology and cell division, as the cells adapted to thrive in the host tissue microenvironment. We also observed the temporary induction of genes characteristic of the host tissue, which was particularly evident when tumor cells were grown on brain tissue. These early transient gene expression events suggest potential points of therapeutic intervention that are not evident in data from well-established tumors. |
format | Online Article Text |
id | pubmed-4086928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40869282014-07-14 Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells Rondeau, Gaelle Abedinpour, Parisa Desai, Prerak Baron, Veronique T. Borgstrom, Per Welsh, John PLoS One Research Article In metastasis, circulating tumor cells penetrate the walls of blood vessels and enter the metastatic target tissue, thereby becoming exposed to novel and relatively unsupportive microenvironments. In the new microenvironments, the tumor cells often remain in a dormant state indefinitely and must adapt before they are able to successfully colonize the tissue. Very little is known about this adaptive process. We studied temporal changes in gene expression when breast cancer cells adapt to survive and grow on brain, bone marrow, and lung tissue maintained in an in vivo culture system, as models of the metastatic colonization of these tissues. We observed the transient activation of genes typically associated with homeostasis and stress during the initial stages of adaptation, followed by the activation of genes that mediate more advanced functions, such as elaboration of cell morphology and cell division, as the cells adapted to thrive in the host tissue microenvironment. We also observed the temporary induction of genes characteristic of the host tissue, which was particularly evident when tumor cells were grown on brain tissue. These early transient gene expression events suggest potential points of therapeutic intervention that are not evident in data from well-established tumors. Public Library of Science 2014-07-08 /pmc/articles/PMC4086928/ /pubmed/25004123 http://dx.doi.org/10.1371/journal.pone.0101160 Text en © 2014 Rondeau et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rondeau, Gaelle Abedinpour, Parisa Desai, Prerak Baron, Veronique T. Borgstrom, Per Welsh, John Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells |
title | Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells |
title_full | Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells |
title_fullStr | Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells |
title_full_unstemmed | Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells |
title_short | Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells |
title_sort | effects of different tissue microenvironments on gene expression in breast cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086928/ https://www.ncbi.nlm.nih.gov/pubmed/25004123 http://dx.doi.org/10.1371/journal.pone.0101160 |
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