A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis

PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting DNA from Map cultured in liquid medium was developed. The proposed protocol (Universidad Austral...

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Detalles Bibliográficos
Autores principales: Salgado, Miguel, Verdugo, Cristobal, Heuer, Cord, Castillo, Pedro, Zamorano, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4087225/
https://www.ncbi.nlm.nih.gov/pubmed/24136213
http://dx.doi.org/10.4142/jvs.2014.15.2.233

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