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Telomeres are elongated in rats exposed to moderate altitude

BACKGROUND: Leukocyte telomere length has been shown to be associated with life span. Hypoxia-associated changes of telomere length have been detected in cell cultures, but no in vivo studies have reported the changes of telomere length under different hypoxic conditions. This study aimed to evaluat...

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Autores principales: Wang, Yaping, Zhou, Wen-di, Yang, Yingzhong, Ma, Lan, Zhao, Yanxia, Bai, Zhenzhong, Ge, Ri-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4088304/
https://www.ncbi.nlm.nih.gov/pubmed/24996852
http://dx.doi.org/10.1186/1880-6805-33-19
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author Wang, Yaping
Zhou, Wen-di
Yang, Yingzhong
Ma, Lan
Zhao, Yanxia
Bai, Zhenzhong
Ge, Ri-Li
author_facet Wang, Yaping
Zhou, Wen-di
Yang, Yingzhong
Ma, Lan
Zhao, Yanxia
Bai, Zhenzhong
Ge, Ri-Li
author_sort Wang, Yaping
collection PubMed
description BACKGROUND: Leukocyte telomere length has been shown to be associated with life span. Hypoxia-associated changes of telomere length have been detected in cell cultures, but no in vivo studies have reported the changes of telomere length under different hypoxic conditions. This study aimed to evaluate the effects of altitude on telomere length in rat leukocytes. METHODS: One hundred and ten male Wistar rats were randomized into 3 groups and maintained at sea-level (altitude of 10 m) (SL group, n = 10), moderate altitude (2,260 m) (MA group, n = 50), or simulated high altitude (5,000 m (SHA group, n = 50). The last two groups were further divided into 5 subgroups and exposed to hypoxia for 1, 3, 7, 15, or 30 days (n = 10). The leukocyte telomere length, hemoglobin concentration, red blood cell count, hematocrit, and plasma levels of telomerase reverse transcriptase (TERT), hypoxia-inducible factor 1α (HIF-1α), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. RESULTS: Leukocyte telomere length was significantly longer in the MA group than in the SL or SHA groups, and the TERT expression changed in a similar manner as the leukocyte telomere length. However, HIF-1α level was significantly higher in both MA and SHA groups than the SL group. SOD level was decreased and MDA level was elevated in SHA group. CONCLUSIONS: The telomere length of blood leukocytes is elongated at a moderate altitude, but not at a high altitude. A mild hypoxic state may increase telomere length.
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spelling pubmed-40883042014-07-10 Telomeres are elongated in rats exposed to moderate altitude Wang, Yaping Zhou, Wen-di Yang, Yingzhong Ma, Lan Zhao, Yanxia Bai, Zhenzhong Ge, Ri-Li J Physiol Anthropol Original Article BACKGROUND: Leukocyte telomere length has been shown to be associated with life span. Hypoxia-associated changes of telomere length have been detected in cell cultures, but no in vivo studies have reported the changes of telomere length under different hypoxic conditions. This study aimed to evaluate the effects of altitude on telomere length in rat leukocytes. METHODS: One hundred and ten male Wistar rats were randomized into 3 groups and maintained at sea-level (altitude of 10 m) (SL group, n = 10), moderate altitude (2,260 m) (MA group, n = 50), or simulated high altitude (5,000 m (SHA group, n = 50). The last two groups were further divided into 5 subgroups and exposed to hypoxia for 1, 3, 7, 15, or 30 days (n = 10). The leukocyte telomere length, hemoglobin concentration, red blood cell count, hematocrit, and plasma levels of telomerase reverse transcriptase (TERT), hypoxia-inducible factor 1α (HIF-1α), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. RESULTS: Leukocyte telomere length was significantly longer in the MA group than in the SL or SHA groups, and the TERT expression changed in a similar manner as the leukocyte telomere length. However, HIF-1α level was significantly higher in both MA and SHA groups than the SL group. SOD level was decreased and MDA level was elevated in SHA group. CONCLUSIONS: The telomere length of blood leukocytes is elongated at a moderate altitude, but not at a high altitude. A mild hypoxic state may increase telomere length. BioMed Central 2014-07-04 /pmc/articles/PMC4088304/ /pubmed/24996852 http://dx.doi.org/10.1186/1880-6805-33-19 Text en Copyright © 2014 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Original Article
Wang, Yaping
Zhou, Wen-di
Yang, Yingzhong
Ma, Lan
Zhao, Yanxia
Bai, Zhenzhong
Ge, Ri-Li
Telomeres are elongated in rats exposed to moderate altitude
title Telomeres are elongated in rats exposed to moderate altitude
title_full Telomeres are elongated in rats exposed to moderate altitude
title_fullStr Telomeres are elongated in rats exposed to moderate altitude
title_full_unstemmed Telomeres are elongated in rats exposed to moderate altitude
title_short Telomeres are elongated in rats exposed to moderate altitude
title_sort telomeres are elongated in rats exposed to moderate altitude
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4088304/
https://www.ncbi.nlm.nih.gov/pubmed/24996852
http://dx.doi.org/10.1186/1880-6805-33-19
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