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Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

A unique open reading frame (ORF) Z3276 was identified as a specific genetic marker for E. coli O157:H7. A qPCR assay was developed for detection of E. coli O157:H7 by targeting ORF Z3276. With this assay, we can detect as low as a few copies of the genome of DNA of E. coli O157:H7. The sensitivity...

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Detalles Bibliográficos
Autores principales:	Li, Baoguang, Hu, Zonglin, Elkins, Christopher A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2014
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4089424/ https://www.ncbi.nlm.nih.gov/pubmed/24513664 http://dx.doi.org/10.3791/50967

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