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Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions

Intercellular cell adhesion molecule-5 (ICAM-5) is a member of the ICAM subfamily that is exclusively expressed in the telencephalon region of the brain. The crystal structure of the four most N-terminal glycosylated domains (D1–D4) of ICAM-5 was determined in three different space groups and the D1...

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Autores principales: Recacha, Rosario, Jiménez, David, Tian, Li, Barredo, Román, Gahmberg, Carl G., Casasnovas, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4089487/
https://www.ncbi.nlm.nih.gov/pubmed/25004970
http://dx.doi.org/10.1107/S1399004714009468
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author Recacha, Rosario
Jiménez, David
Tian, Li
Barredo, Román
Gahmberg, Carl G.
Casasnovas, José M.
author_facet Recacha, Rosario
Jiménez, David
Tian, Li
Barredo, Román
Gahmberg, Carl G.
Casasnovas, José M.
author_sort Recacha, Rosario
collection PubMed
description Intercellular cell adhesion molecule-5 (ICAM-5) is a member of the ICAM subfamily that is exclusively expressed in the telencephalon region of the brain. The crystal structure of the four most N-terminal glycosylated domains (D1–D4) of ICAM-5 was determined in three different space groups and the D1–D5 fragment was modelled. The structures showed a curved molecule with two pronounced interdomain bends between D2 and D3 and between D3 and D4, as well as some interdomain flexibility. In contrast to ICAM-1, ICAM-5 has patches of positive and negative electrostatic charge at D1–D2 and at D3–D5, respectively. ICAM-5 can mediate homotypic interactions. In the crystals, several charge-based intermolecular interactions between the N-terminal and C-terminal moieties of the ICAM-5 molecules were observed, which defined an interacting surface in the D1–D4 fragment. One of the crystal lattices has a molecular assembly that could represent the homophilic ICAM-5 cell adhesion complex in neurons.
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spelling pubmed-40894872014-07-16 Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions Recacha, Rosario Jiménez, David Tian, Li Barredo, Román Gahmberg, Carl G. Casasnovas, José M. Acta Crystallogr D Biol Crystallogr Research Papers Intercellular cell adhesion molecule-5 (ICAM-5) is a member of the ICAM subfamily that is exclusively expressed in the telencephalon region of the brain. The crystal structure of the four most N-terminal glycosylated domains (D1–D4) of ICAM-5 was determined in three different space groups and the D1–D5 fragment was modelled. The structures showed a curved molecule with two pronounced interdomain bends between D2 and D3 and between D3 and D4, as well as some interdomain flexibility. In contrast to ICAM-1, ICAM-5 has patches of positive and negative electrostatic charge at D1–D2 and at D3–D5, respectively. ICAM-5 can mediate homotypic interactions. In the crystals, several charge-based intermolecular interactions between the N-terminal and C-terminal moieties of the ICAM-5 molecules were observed, which defined an interacting surface in the D1–D4 fragment. One of the crystal lattices has a molecular assembly that could represent the homophilic ICAM-5 cell adhesion complex in neurons. International Union of Crystallography 2014-06-29 /pmc/articles/PMC4089487/ /pubmed/25004970 http://dx.doi.org/10.1107/S1399004714009468 Text en © Recacha et al. 2014 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Recacha, Rosario
Jiménez, David
Tian, Li
Barredo, Román
Gahmberg, Carl G.
Casasnovas, José M.
Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions
title Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions
title_full Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions
title_fullStr Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions
title_full_unstemmed Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions
title_short Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions
title_sort crystal structures of an icam-5 ectodomain fragment show electrostatic-based homophilic adhesions
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4089487/
https://www.ncbi.nlm.nih.gov/pubmed/25004970
http://dx.doi.org/10.1107/S1399004714009468
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