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Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions
Intercellular cell adhesion molecule-5 (ICAM-5) is a member of the ICAM subfamily that is exclusively expressed in the telencephalon region of the brain. The crystal structure of the four most N-terminal glycosylated domains (D1–D4) of ICAM-5 was determined in three different space groups and the D1...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4089487/ https://www.ncbi.nlm.nih.gov/pubmed/25004970 http://dx.doi.org/10.1107/S1399004714009468 |
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author | Recacha, Rosario Jiménez, David Tian, Li Barredo, Román Gahmberg, Carl G. Casasnovas, José M. |
author_facet | Recacha, Rosario Jiménez, David Tian, Li Barredo, Román Gahmberg, Carl G. Casasnovas, José M. |
author_sort | Recacha, Rosario |
collection | PubMed |
description | Intercellular cell adhesion molecule-5 (ICAM-5) is a member of the ICAM subfamily that is exclusively expressed in the telencephalon region of the brain. The crystal structure of the four most N-terminal glycosylated domains (D1–D4) of ICAM-5 was determined in three different space groups and the D1–D5 fragment was modelled. The structures showed a curved molecule with two pronounced interdomain bends between D2 and D3 and between D3 and D4, as well as some interdomain flexibility. In contrast to ICAM-1, ICAM-5 has patches of positive and negative electrostatic charge at D1–D2 and at D3–D5, respectively. ICAM-5 can mediate homotypic interactions. In the crystals, several charge-based intermolecular interactions between the N-terminal and C-terminal moieties of the ICAM-5 molecules were observed, which defined an interacting surface in the D1–D4 fragment. One of the crystal lattices has a molecular assembly that could represent the homophilic ICAM-5 cell adhesion complex in neurons. |
format | Online Article Text |
id | pubmed-4089487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-40894872014-07-16 Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions Recacha, Rosario Jiménez, David Tian, Li Barredo, Román Gahmberg, Carl G. Casasnovas, José M. Acta Crystallogr D Biol Crystallogr Research Papers Intercellular cell adhesion molecule-5 (ICAM-5) is a member of the ICAM subfamily that is exclusively expressed in the telencephalon region of the brain. The crystal structure of the four most N-terminal glycosylated domains (D1–D4) of ICAM-5 was determined in three different space groups and the D1–D5 fragment was modelled. The structures showed a curved molecule with two pronounced interdomain bends between D2 and D3 and between D3 and D4, as well as some interdomain flexibility. In contrast to ICAM-1, ICAM-5 has patches of positive and negative electrostatic charge at D1–D2 and at D3–D5, respectively. ICAM-5 can mediate homotypic interactions. In the crystals, several charge-based intermolecular interactions between the N-terminal and C-terminal moieties of the ICAM-5 molecules were observed, which defined an interacting surface in the D1–D4 fragment. One of the crystal lattices has a molecular assembly that could represent the homophilic ICAM-5 cell adhesion complex in neurons. International Union of Crystallography 2014-06-29 /pmc/articles/PMC4089487/ /pubmed/25004970 http://dx.doi.org/10.1107/S1399004714009468 Text en © Recacha et al. 2014 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Recacha, Rosario Jiménez, David Tian, Li Barredo, Román Gahmberg, Carl G. Casasnovas, José M. Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions |
title | Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions |
title_full | Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions |
title_fullStr | Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions |
title_full_unstemmed | Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions |
title_short | Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions |
title_sort | crystal structures of an icam-5 ectodomain fragment show electrostatic-based homophilic adhesions |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4089487/ https://www.ncbi.nlm.nih.gov/pubmed/25004970 http://dx.doi.org/10.1107/S1399004714009468 |
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