Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

Monomeric CRISPR-Cas9 nucleases are widely used for targeted genome editing but can induce unwanted off-target mutations with high frequencies. Here we describe dimeric RNA-guided FokI Nucleases (RFNs) that recognize extended sequences and can edit endogenous genes with high efficiencies in human ce...

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Detalles Bibliográficos
Autores principales: Tsai, Shengdar Q., Wyvekens, Nicolas, Khayter, Cyd, Foden, Jennifer A., Thapar, Vishal, Reyon, Deepak, Goodwin, Mathew J., Aryee, Martin J., Joung, J. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090141/
https://www.ncbi.nlm.nih.gov/pubmed/24770325
http://dx.doi.org/10.1038/nbt.2908

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090141/
https://www.ncbi.nlm.nih.gov/pubmed/24770325
http://dx.doi.org/10.1038/nbt.2908

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