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Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

Monomeric CRISPR-Cas9 nucleases are widely used for targeted genome editing but can induce unwanted off-target mutations with high frequencies. Here we describe dimeric RNA-guided FokI Nucleases (RFNs) that recognize extended sequences and can edit endogenous genes with high efficiencies in human ce...

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Detalles Bibliográficos
Autores principales:	Tsai, Shengdar Q., Wyvekens, Nicolas, Khayter, Cyd, Foden, Jennifer A., Thapar, Vishal, Reyon, Deepak, Goodwin, Mathew J., Aryee, Martin J., Joung, J. Keith
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090141/ https://www.ncbi.nlm.nih.gov/pubmed/24770325 http://dx.doi.org/10.1038/nbt.2908

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