Cargando…

A Polymer-Based Magnetic Resonance Tracer for Visualization of Solid Tumors by (13)C Spectroscopic Imaging

Morphological imaging precedes lesion-specific visualization in magnetic resonance imaging (MRI) because of the superior ability of this technique to depict tissue morphology with excellent spatial and temporal resolutions. To achieve lesion-specific visualization of tumors by MRI, we investigated t...

Descripción completa

Detalles Bibliográficos
Autores principales: Suzuki, Yoshikazu, Iida, Mitsuru, Miura, Iwao, Inubushi, Toshiro, Morikawa, Shigehiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090184/
https://www.ncbi.nlm.nih.gov/pubmed/25007334
http://dx.doi.org/10.1371/journal.pone.0102132
_version_ 1782480599081025536
author Suzuki, Yoshikazu
Iida, Mitsuru
Miura, Iwao
Inubushi, Toshiro
Morikawa, Shigehiro
author_facet Suzuki, Yoshikazu
Iida, Mitsuru
Miura, Iwao
Inubushi, Toshiro
Morikawa, Shigehiro
author_sort Suzuki, Yoshikazu
collection PubMed
description Morphological imaging precedes lesion-specific visualization in magnetic resonance imaging (MRI) because of the superior ability of this technique to depict tissue morphology with excellent spatial and temporal resolutions. To achieve lesion-specific visualization of tumors by MRI, we investigated the availability of a novel polymer-based tracer. Although the (13)C nucleus is a candidate for a detection nucleus because of its low background signal in the body, the low magnetic resonance sensitivity of the nucleus needs to be resolved before developing a (13)C-based tracer. In order to overcome this problem, we enriched polyethylene glycol (PEG), a biocompatible polymer, with (13)C atoms. (13)C-PEG40,000 ((13)C-PEG with an average molecular weight of 40 kDa) emitted a single (13)C signal with a high signal-to-noise ratio due to its ability to maintain signal sharpness, as was confirmed by in vivo investigation, and displayed a chemical shift sufficiently distinct from that of endogenous fat. (13)C-PEG40,000 intravenously injected into mice showed long retention in circulation, leading to its effective accumulation in tumors reflecting the well-known phenomenon that macromolecules accumulate in tumors because of leaky tumor capillaries. These properties of (13)C-PEG40,000 allowed visualization of tumors in mice by (13)C spectroscopic imaging. These findings suggest that a technique based on (13)C-PEG is a promising strategy for tumor detection.
format Online
Article
Text
id pubmed-4090184
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-40901842014-07-14 A Polymer-Based Magnetic Resonance Tracer for Visualization of Solid Tumors by (13)C Spectroscopic Imaging Suzuki, Yoshikazu Iida, Mitsuru Miura, Iwao Inubushi, Toshiro Morikawa, Shigehiro PLoS One Research Article Morphological imaging precedes lesion-specific visualization in magnetic resonance imaging (MRI) because of the superior ability of this technique to depict tissue morphology with excellent spatial and temporal resolutions. To achieve lesion-specific visualization of tumors by MRI, we investigated the availability of a novel polymer-based tracer. Although the (13)C nucleus is a candidate for a detection nucleus because of its low background signal in the body, the low magnetic resonance sensitivity of the nucleus needs to be resolved before developing a (13)C-based tracer. In order to overcome this problem, we enriched polyethylene glycol (PEG), a biocompatible polymer, with (13)C atoms. (13)C-PEG40,000 ((13)C-PEG with an average molecular weight of 40 kDa) emitted a single (13)C signal with a high signal-to-noise ratio due to its ability to maintain signal sharpness, as was confirmed by in vivo investigation, and displayed a chemical shift sufficiently distinct from that of endogenous fat. (13)C-PEG40,000 intravenously injected into mice showed long retention in circulation, leading to its effective accumulation in tumors reflecting the well-known phenomenon that macromolecules accumulate in tumors because of leaky tumor capillaries. These properties of (13)C-PEG40,000 allowed visualization of tumors in mice by (13)C spectroscopic imaging. These findings suggest that a technique based on (13)C-PEG is a promising strategy for tumor detection. Public Library of Science 2014-07-09 /pmc/articles/PMC4090184/ /pubmed/25007334 http://dx.doi.org/10.1371/journal.pone.0102132 Text en © 2014 Suzuki et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Suzuki, Yoshikazu
Iida, Mitsuru
Miura, Iwao
Inubushi, Toshiro
Morikawa, Shigehiro
A Polymer-Based Magnetic Resonance Tracer for Visualization of Solid Tumors by (13)C Spectroscopic Imaging
title A Polymer-Based Magnetic Resonance Tracer for Visualization of Solid Tumors by (13)C Spectroscopic Imaging
title_full A Polymer-Based Magnetic Resonance Tracer for Visualization of Solid Tumors by (13)C Spectroscopic Imaging
title_fullStr A Polymer-Based Magnetic Resonance Tracer for Visualization of Solid Tumors by (13)C Spectroscopic Imaging
title_full_unstemmed A Polymer-Based Magnetic Resonance Tracer for Visualization of Solid Tumors by (13)C Spectroscopic Imaging
title_short A Polymer-Based Magnetic Resonance Tracer for Visualization of Solid Tumors by (13)C Spectroscopic Imaging
title_sort polymer-based magnetic resonance tracer for visualization of solid tumors by (13)c spectroscopic imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090184/
https://www.ncbi.nlm.nih.gov/pubmed/25007334
http://dx.doi.org/10.1371/journal.pone.0102132
work_keys_str_mv AT suzukiyoshikazu apolymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT iidamitsuru apolymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT miuraiwao apolymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT inubushitoshiro apolymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT morikawashigehiro apolymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT suzukiyoshikazu polymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT iidamitsuru polymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT miuraiwao polymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT inubushitoshiro polymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging
AT morikawashigehiro polymerbasedmagneticresonancetracerforvisualizationofsolidtumorsby13cspectroscopicimaging