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In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration
Despite current treatment regimens, heart failure remains the leading cause of morbidity and mortality in the developed world due to the limited capacity of adult mammalian ventricular cardiomyocytes to divide and replace ventricular myocardium lost from ischemia-induced infarct(1,2). As a result, t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090927/ https://www.ncbi.nlm.nih.gov/pubmed/23783515 http://dx.doi.org/10.1038/nature12322 |
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author | Zhang, Ruilin Han, Peidong Yang, Hongbo Ouyang, Kunfu Lee, Derek Lin, Yi-Fan Ocorr, Karen Kang, Guson Chen, Ju Stainier, Didier Y.R. Yelon, Deborah Chi, Neil C. |
author_facet | Zhang, Ruilin Han, Peidong Yang, Hongbo Ouyang, Kunfu Lee, Derek Lin, Yi-Fan Ocorr, Karen Kang, Guson Chen, Ju Stainier, Didier Y.R. Yelon, Deborah Chi, Neil C. |
author_sort | Zhang, Ruilin |
collection | PubMed |
description | Despite current treatment regimens, heart failure remains the leading cause of morbidity and mortality in the developed world due to the limited capacity of adult mammalian ventricular cardiomyocytes to divide and replace ventricular myocardium lost from ischemia-induced infarct(1,2). As a result, there is great interest to identify potential cellular sources and strategies to generate new ventricular myocardium(3). Past studies have shown that lower vertebrate and early postnatal mammalian ventricular cardiomyocytes can proliferate to help regenerate injured ventricles(4–6); however, recent studies have suggested that additional endogenous cellular sources may contribute to this overall ventricular regeneration(3). Here, we have developed in the zebrafish a combination of fluorescent reporter transgenes, genetic fate-mapping strategies, and a ventricle-specific genetic ablation system to discover that differentiated atrial cardiomyocytes can transdifferentiate into ventricular cardiomyocytes to contribute to zebrafish cardiac ventricular regeneration. Using in vivo time-lapse and confocal imaging, we monitored the dynamic cellular events during atrial-to-ventricular cardiomyocyte transdifferentiation to define intermediate cardiac reprogramming stages. Importantly, we observed that Notch signaling becomes activated in the atrial endocardium following ventricular ablation, and discovered that inhibiting Notch signaling blocked the atrial-to-ventricular transdifferentiation and cardiac regeneration. Overall, these studies not only provide evidence for the plasticity of cardiac lineages during myocardial injury, but more importantly reveal an abundant new potential cardiac resident cellular source for cardiac ventricular regeneration. |
format | Online Article Text |
id | pubmed-4090927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-40909272014-07-10 In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration Zhang, Ruilin Han, Peidong Yang, Hongbo Ouyang, Kunfu Lee, Derek Lin, Yi-Fan Ocorr, Karen Kang, Guson Chen, Ju Stainier, Didier Y.R. Yelon, Deborah Chi, Neil C. Nature Article Despite current treatment regimens, heart failure remains the leading cause of morbidity and mortality in the developed world due to the limited capacity of adult mammalian ventricular cardiomyocytes to divide and replace ventricular myocardium lost from ischemia-induced infarct(1,2). As a result, there is great interest to identify potential cellular sources and strategies to generate new ventricular myocardium(3). Past studies have shown that lower vertebrate and early postnatal mammalian ventricular cardiomyocytes can proliferate to help regenerate injured ventricles(4–6); however, recent studies have suggested that additional endogenous cellular sources may contribute to this overall ventricular regeneration(3). Here, we have developed in the zebrafish a combination of fluorescent reporter transgenes, genetic fate-mapping strategies, and a ventricle-specific genetic ablation system to discover that differentiated atrial cardiomyocytes can transdifferentiate into ventricular cardiomyocytes to contribute to zebrafish cardiac ventricular regeneration. Using in vivo time-lapse and confocal imaging, we monitored the dynamic cellular events during atrial-to-ventricular cardiomyocyte transdifferentiation to define intermediate cardiac reprogramming stages. Importantly, we observed that Notch signaling becomes activated in the atrial endocardium following ventricular ablation, and discovered that inhibiting Notch signaling blocked the atrial-to-ventricular transdifferentiation and cardiac regeneration. Overall, these studies not only provide evidence for the plasticity of cardiac lineages during myocardial injury, but more importantly reveal an abundant new potential cardiac resident cellular source for cardiac ventricular regeneration. 2013-06-19 2013-06-27 /pmc/articles/PMC4090927/ /pubmed/23783515 http://dx.doi.org/10.1038/nature12322 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Zhang, Ruilin Han, Peidong Yang, Hongbo Ouyang, Kunfu Lee, Derek Lin, Yi-Fan Ocorr, Karen Kang, Guson Chen, Ju Stainier, Didier Y.R. Yelon, Deborah Chi, Neil C. In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration |
title | In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration |
title_full | In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration |
title_fullStr | In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration |
title_full_unstemmed | In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration |
title_short | In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration |
title_sort | in vivo cardiac reprogramming contributes to zebrafish heart regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090927/ https://www.ncbi.nlm.nih.gov/pubmed/23783515 http://dx.doi.org/10.1038/nature12322 |
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