Cargando…
Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs
Nitrogen is considered the most limiting nutrient for maize (Zea mays L.), but there is limited understanding of the regulation of nitrogen-related genes during maize development. An Affymetrix 82K maize array was used to analyze the expression of ≤ 46 unique nitrogen uptake and assimilation probes...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091066/ https://www.ncbi.nlm.nih.gov/pubmed/24270626 http://dx.doi.org/10.4161/psb.26056 |
_version_ | 1782480732145319936 |
---|---|
author | Liseron-Monfils, Christophe Bi, Yong-Mei Downs, Gregory S Wu, Wenqing Signorelli, Tara Lu, Guangwen Chen, Xi Bondo, Eddie Zhu, Tong Lukens, Lewis N Colasanti, Joseph Rothstein, Steven J Raizada, Manish N |
author_facet | Liseron-Monfils, Christophe Bi, Yong-Mei Downs, Gregory S Wu, Wenqing Signorelli, Tara Lu, Guangwen Chen, Xi Bondo, Eddie Zhu, Tong Lukens, Lewis N Colasanti, Joseph Rothstein, Steven J Raizada, Manish N |
author_sort | Liseron-Monfils, Christophe |
collection | PubMed |
description | Nitrogen is considered the most limiting nutrient for maize (Zea mays L.), but there is limited understanding of the regulation of nitrogen-related genes during maize development. An Affymetrix 82K maize array was used to analyze the expression of ≤ 46 unique nitrogen uptake and assimilation probes in 50 maize tissues from seedling emergence to 31 d after pollination. Four nitrogen-related expression clusters were identified in roots and shoots corresponding to, or overlapping, juvenile, adult, and reproductive phases of development. Quantitative real time PCR data was consistent with the existence of these distinct expression clusters. Promoters corresponding to each cluster were screened for over-represented cis-acting elements. The 8-bp distal motif of the Arabidopsis 43-bp nitrogen response element (NRE) was over-represented in nitrogen-related maize gene promoters. This conserved motif, referred to here as NRE43-d8, was previously shown to be critical for nitrate-activated transcription of nitrate reductase (NIA1) and nitrite reductase (NIR1) by the NIN-LIKE PROTEIN 6 (NLP6) in Arabidopsis. Here, NRE43-d8 was over-represented in the promoters of maize nitrate and ammonium transporter genes, specifically those that showed peak expression during early-stage vegetative development. This result predicts an expansion of the NRE-NLP6 regulon and suggests that it may have a developmental component in maize. We also report leaf expression of putative orthologs of nitrite transporters (NiTR1), a transporter not previously reported in maize. We conclude by discussing how each of the four transcriptional modules may be responsible for the different nitrogen uptake and assimilation requirements of leaves and roots at different stages of maize development. |
format | Online Article Text |
id | pubmed-4091066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-40910662014-07-18 Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs Liseron-Monfils, Christophe Bi, Yong-Mei Downs, Gregory S Wu, Wenqing Signorelli, Tara Lu, Guangwen Chen, Xi Bondo, Eddie Zhu, Tong Lukens, Lewis N Colasanti, Joseph Rothstein, Steven J Raizada, Manish N Plant Signal Behav Research Paper Nitrogen is considered the most limiting nutrient for maize (Zea mays L.), but there is limited understanding of the regulation of nitrogen-related genes during maize development. An Affymetrix 82K maize array was used to analyze the expression of ≤ 46 unique nitrogen uptake and assimilation probes in 50 maize tissues from seedling emergence to 31 d after pollination. Four nitrogen-related expression clusters were identified in roots and shoots corresponding to, or overlapping, juvenile, adult, and reproductive phases of development. Quantitative real time PCR data was consistent with the existence of these distinct expression clusters. Promoters corresponding to each cluster were screened for over-represented cis-acting elements. The 8-bp distal motif of the Arabidopsis 43-bp nitrogen response element (NRE) was over-represented in nitrogen-related maize gene promoters. This conserved motif, referred to here as NRE43-d8, was previously shown to be critical for nitrate-activated transcription of nitrate reductase (NIA1) and nitrite reductase (NIR1) by the NIN-LIKE PROTEIN 6 (NLP6) in Arabidopsis. Here, NRE43-d8 was over-represented in the promoters of maize nitrate and ammonium transporter genes, specifically those that showed peak expression during early-stage vegetative development. This result predicts an expansion of the NRE-NLP6 regulon and suggests that it may have a developmental component in maize. We also report leaf expression of putative orthologs of nitrite transporters (NiTR1), a transporter not previously reported in maize. We conclude by discussing how each of the four transcriptional modules may be responsible for the different nitrogen uptake and assimilation requirements of leaves and roots at different stages of maize development. Landes Bioscience 2013-08-20 /pmc/articles/PMC4091066/ /pubmed/24270626 http://dx.doi.org/10.4161/psb.26056 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Research Paper Liseron-Monfils, Christophe Bi, Yong-Mei Downs, Gregory S Wu, Wenqing Signorelli, Tara Lu, Guangwen Chen, Xi Bondo, Eddie Zhu, Tong Lukens, Lewis N Colasanti, Joseph Rothstein, Steven J Raizada, Manish N Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs |
title | Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs |
title_full | Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs |
title_fullStr | Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs |
title_full_unstemmed | Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs |
title_short | Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs |
title_sort | nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (zea mays l.) associated with distinct cis-acting promoter motifs |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091066/ https://www.ncbi.nlm.nih.gov/pubmed/24270626 http://dx.doi.org/10.4161/psb.26056 |
work_keys_str_mv | AT liseronmonfilschristophe nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT biyongmei nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT downsgregorys nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT wuwenqing nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT signorellitara nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT luguangwen nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT chenxi nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT bondoeddie nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT zhutong nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT lukenslewisn nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT colasantijoseph nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT rothsteinstevenj nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs AT raizadamanishn nitrogentransporterandassimilationgenesexhibitdevelopmentalstageselectiveexpressioninmaizezeamayslassociatedwithdistinctcisactingpromotermotifs |