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Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutumL. cv Khandwa-2)

Embryogenesis in cotton is a difficult task due its genome dependency. We used 3 cotton cultivars (Khandwa-2, G. Cot. 10, and BC-68–2) and Coker-312 as control for regeneration. Efficient somatic embryogenesis was induced in agronomically important Indian cotton cultivars, Khandwa-2 and G. Cot. 10....

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Autores principales: Kumar, Manoj, Singh, Harpal, Shukla, Anoop Kumar, Verma, Praveen Chandra, Singh, Pradhyumna Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091074/
https://www.ncbi.nlm.nih.gov/pubmed/24494238
http://dx.doi.org/10.4161/psb.26762
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author Kumar, Manoj
Singh, Harpal
Shukla, Anoop Kumar
Verma, Praveen Chandra
Singh, Pradhyumna Kumar
author_facet Kumar, Manoj
Singh, Harpal
Shukla, Anoop Kumar
Verma, Praveen Chandra
Singh, Pradhyumna Kumar
author_sort Kumar, Manoj
collection PubMed
description Embryogenesis in cotton is a difficult task due its genome dependency. We used 3 cotton cultivars (Khandwa-2, G. Cot. 10, and BC-68–2) and Coker-312 as control for regeneration. Efficient somatic embryogenesis was induced in agronomically important Indian cotton cultivars, Khandwa-2 and G. Cot. 10. For callusing in all the cultivars, different media combinations were tried. Embryogenesis was initiated on a hormone-free MS medium (MSB). For embryo maturation and recovery excess of L-glutamine and l-asparagine were used. Khandwa-2 somatic embryos were successfully regenerated into plants. However, no plantlet was obtained in case of G. Cot. 10. Callus induction was also observed in BC-68–2 but there was no embryogenesis observed. The study indicated that the medium and genotype significantly effects embryogenesis. An efficient protocol is described here for regenerating plants via somatic embryogenesis in an elite Indian cotton cultivar Khandwa-2.
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spelling pubmed-40910742014-07-18 Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutumL. cv Khandwa-2) Kumar, Manoj Singh, Harpal Shukla, Anoop Kumar Verma, Praveen Chandra Singh, Pradhyumna Kumar Plant Signal Behav Research Paper Embryogenesis in cotton is a difficult task due its genome dependency. We used 3 cotton cultivars (Khandwa-2, G. Cot. 10, and BC-68–2) and Coker-312 as control for regeneration. Efficient somatic embryogenesis was induced in agronomically important Indian cotton cultivars, Khandwa-2 and G. Cot. 10. For callusing in all the cultivars, different media combinations were tried. Embryogenesis was initiated on a hormone-free MS medium (MSB). For embryo maturation and recovery excess of L-glutamine and l-asparagine were used. Khandwa-2 somatic embryos were successfully regenerated into plants. However, no plantlet was obtained in case of G. Cot. 10. Callus induction was also observed in BC-68–2 but there was no embryogenesis observed. The study indicated that the medium and genotype significantly effects embryogenesis. An efficient protocol is described here for regenerating plants via somatic embryogenesis in an elite Indian cotton cultivar Khandwa-2. Landes Bioscience 2013-10-29 /pmc/articles/PMC4091074/ /pubmed/24494238 http://dx.doi.org/10.4161/psb.26762 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Research Paper
Kumar, Manoj
Singh, Harpal
Shukla, Anoop Kumar
Verma, Praveen Chandra
Singh, Pradhyumna Kumar
Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutumL. cv Khandwa-2)
title Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutumL. cv Khandwa-2)
title_full Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutumL. cv Khandwa-2)
title_fullStr Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutumL. cv Khandwa-2)
title_full_unstemmed Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutumL. cv Khandwa-2)
title_short Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutumL. cv Khandwa-2)
title_sort induction and establishment of somatic embryogenesis in elite indian cotton cultivar (gossypium hirsutuml. cv khandwa-2)
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091074/
https://www.ncbi.nlm.nih.gov/pubmed/24494238
http://dx.doi.org/10.4161/psb.26762
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