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Transcription regulation during stable elongation by a reversible halt of RNA polymerase II

Regulation of RNA polymerase II (RNAPII) during transcription is essential for controlling gene expression. Here we report that the transcriptional activity of RNAPII at the Balbiani ring 2.1 gene could be halted during stable elongation in salivary gland cells of Chironomus tentans larvae for exten...

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Autores principales: Kaminski, Tim Patrick, Siebrasse, Jan Peter, Kubitscheck, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091832/
https://www.ncbi.nlm.nih.gov/pubmed/24850889
http://dx.doi.org/10.1091/mbc.E14-02-0755
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author Kaminski, Tim Patrick
Siebrasse, Jan Peter
Kubitscheck, Ulrich
author_facet Kaminski, Tim Patrick
Siebrasse, Jan Peter
Kubitscheck, Ulrich
author_sort Kaminski, Tim Patrick
collection PubMed
description Regulation of RNA polymerase II (RNAPII) during transcription is essential for controlling gene expression. Here we report that the transcriptional activity of RNAPII at the Balbiani ring 2.1 gene could be halted during stable elongation in salivary gland cells of Chironomus tentans larvae for extended time periods in a regulated manner. The transcription halt was triggered by heat shock and affected all RNAPII independently of their position in the gene. During the halt, incomplete transcripts and RNAPII remained at the transcription site, the phosphorylation state of RNAPII was unaltered, and the transcription bubbles remained open. The transcription of halted transcripts was resumed upon relief of the heat shock. The observed mechanism allows cells to interrupt transcription for extended time periods and rapidly reactivate it without the need to reinitiate transcription of the complete gene. Our results suggest a so-far-unknown level of transcriptional control in eukaryotic cells.
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spelling pubmed-40918322014-10-01 Transcription regulation during stable elongation by a reversible halt of RNA polymerase II Kaminski, Tim Patrick Siebrasse, Jan Peter Kubitscheck, Ulrich Mol Biol Cell Articles Regulation of RNA polymerase II (RNAPII) during transcription is essential for controlling gene expression. Here we report that the transcriptional activity of RNAPII at the Balbiani ring 2.1 gene could be halted during stable elongation in salivary gland cells of Chironomus tentans larvae for extended time periods in a regulated manner. The transcription halt was triggered by heat shock and affected all RNAPII independently of their position in the gene. During the halt, incomplete transcripts and RNAPII remained at the transcription site, the phosphorylation state of RNAPII was unaltered, and the transcription bubbles remained open. The transcription of halted transcripts was resumed upon relief of the heat shock. The observed mechanism allows cells to interrupt transcription for extended time periods and rapidly reactivate it without the need to reinitiate transcription of the complete gene. Our results suggest a so-far-unknown level of transcriptional control in eukaryotic cells. The American Society for Cell Biology 2014-07-15 /pmc/articles/PMC4091832/ /pubmed/24850889 http://dx.doi.org/10.1091/mbc.E14-02-0755 Text en © 2014 Kaminski et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Kaminski, Tim Patrick
Siebrasse, Jan Peter
Kubitscheck, Ulrich
Transcription regulation during stable elongation by a reversible halt of RNA polymerase II
title Transcription regulation during stable elongation by a reversible halt of RNA polymerase II
title_full Transcription regulation during stable elongation by a reversible halt of RNA polymerase II
title_fullStr Transcription regulation during stable elongation by a reversible halt of RNA polymerase II
title_full_unstemmed Transcription regulation during stable elongation by a reversible halt of RNA polymerase II
title_short Transcription regulation during stable elongation by a reversible halt of RNA polymerase II
title_sort transcription regulation during stable elongation by a reversible halt of rna polymerase ii
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091832/
https://www.ncbi.nlm.nih.gov/pubmed/24850889
http://dx.doi.org/10.1091/mbc.E14-02-0755
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