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Super-Resolution Microscopy Using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions
[Image: see text] We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K). Given the limit imposed by the lack of cryo-immersion objectives, curr...
Autores principales: | Kaufmann, Rainer, Schellenberger, Pascale, Seiradake, Elena, Dobbie, Ian M., Jones, E. Yvonne, Davis, Ilan, Hagen, Christoph, Grünewald, Kay |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4092024/ https://www.ncbi.nlm.nih.gov/pubmed/24884378 http://dx.doi.org/10.1021/nl501870p |
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