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Effect of photoperiod on the feline adipose transcriptome as assessed by RNA sequencing

BACKGROUND: Photoperiod is known to cause physiological changes in seasonal mammals, including changes in body weight, physical activity, reproductive status, and adipose tissue gene expression in several species. The objective of this study was to determine the effects of day length on the adipose...

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Autores principales: Mori, Akihiro, Kappen, Kelly L, Dilger, Anna C, Swanson, Kelly S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4092351/
https://www.ncbi.nlm.nih.gov/pubmed/24992939
http://dx.doi.org/10.1186/1746-6148-10-146
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author Mori, Akihiro
Kappen, Kelly L
Dilger, Anna C
Swanson, Kelly S
author_facet Mori, Akihiro
Kappen, Kelly L
Dilger, Anna C
Swanson, Kelly S
author_sort Mori, Akihiro
collection PubMed
description BACKGROUND: Photoperiod is known to cause physiological changes in seasonal mammals, including changes in body weight, physical activity, reproductive status, and adipose tissue gene expression in several species. The objective of this study was to determine the effects of day length on the adipose transcriptome of cats as assessed by RNA sequencing. Ten healthy adult neutered male domestic shorthair cats were used in a randomized crossover design study. During two 12-wk periods, cats were exposed to either short days (8 hr light:16 hr dark) or long days (16 hr light:8 hr dark). Cats were fed a commercial diet to maintain baseline body weight to avoid weight-related bias. Subcutaneous adipose biopsies were collected at wk 12 of each period for RNA isolation and sequencing. RESULTS: A total of 578 million sequences (28.9 million/sample) were generated by Illumina sequencing. A total of 170 mRNA transcripts were differentially expressed between short day- and long day-housed cats. 89 annotated transcripts were up-regulated by short days, while 24 annotated transcripts were down-regulated by short days. Another 57 un-annotated transcripts were also different between groups. Adipose tissue of short day-housed cats had greater expression of genes involved with cell growth and differentiation (e.g., myostatin; frizzled-related protein), cell development and structure (e.g., cytokeratins), and protein processing and ubiquitination (e.g., kelch-like proteins). In contrast, short day-housed cats had decreased expression of genes involved with immune function (e.g., plasminogen activator inhibitor 1; chemokine (C-C motif) ligand 2; C-C motif chemokine 5; T-cell activators), and altered expression of genes associated with carbohydrate and lipid metabolism. CONCLUSIONS: Collectively, these gene expression changes suggest that short day housing may promote adipogenesis, minimize inflammation and oxidative stress, and alter nutrient metabolism in feline adipose tissue, even when fed to maintain body weight. Although this study has highlighted molecular mechanisms contributing to the seasonal metabolic changes observed in cats, future research that specifically targets and studies these biological pathways, and the physiological outcomes that are affected by them, is justified.
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spelling pubmed-40923512014-07-12 Effect of photoperiod on the feline adipose transcriptome as assessed by RNA sequencing Mori, Akihiro Kappen, Kelly L Dilger, Anna C Swanson, Kelly S BMC Vet Res Research Article BACKGROUND: Photoperiod is known to cause physiological changes in seasonal mammals, including changes in body weight, physical activity, reproductive status, and adipose tissue gene expression in several species. The objective of this study was to determine the effects of day length on the adipose transcriptome of cats as assessed by RNA sequencing. Ten healthy adult neutered male domestic shorthair cats were used in a randomized crossover design study. During two 12-wk periods, cats were exposed to either short days (8 hr light:16 hr dark) or long days (16 hr light:8 hr dark). Cats were fed a commercial diet to maintain baseline body weight to avoid weight-related bias. Subcutaneous adipose biopsies were collected at wk 12 of each period for RNA isolation and sequencing. RESULTS: A total of 578 million sequences (28.9 million/sample) were generated by Illumina sequencing. A total of 170 mRNA transcripts were differentially expressed between short day- and long day-housed cats. 89 annotated transcripts were up-regulated by short days, while 24 annotated transcripts were down-regulated by short days. Another 57 un-annotated transcripts were also different between groups. Adipose tissue of short day-housed cats had greater expression of genes involved with cell growth and differentiation (e.g., myostatin; frizzled-related protein), cell development and structure (e.g., cytokeratins), and protein processing and ubiquitination (e.g., kelch-like proteins). In contrast, short day-housed cats had decreased expression of genes involved with immune function (e.g., plasminogen activator inhibitor 1; chemokine (C-C motif) ligand 2; C-C motif chemokine 5; T-cell activators), and altered expression of genes associated with carbohydrate and lipid metabolism. CONCLUSIONS: Collectively, these gene expression changes suggest that short day housing may promote adipogenesis, minimize inflammation and oxidative stress, and alter nutrient metabolism in feline adipose tissue, even when fed to maintain body weight. Although this study has highlighted molecular mechanisms contributing to the seasonal metabolic changes observed in cats, future research that specifically targets and studies these biological pathways, and the physiological outcomes that are affected by them, is justified. BioMed Central 2014-07-03 /pmc/articles/PMC4092351/ /pubmed/24992939 http://dx.doi.org/10.1186/1746-6148-10-146 Text en Copyright © 2014 Mori et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Mori, Akihiro
Kappen, Kelly L
Dilger, Anna C
Swanson, Kelly S
Effect of photoperiod on the feline adipose transcriptome as assessed by RNA sequencing
title Effect of photoperiod on the feline adipose transcriptome as assessed by RNA sequencing
title_full Effect of photoperiod on the feline adipose transcriptome as assessed by RNA sequencing
title_fullStr Effect of photoperiod on the feline adipose transcriptome as assessed by RNA sequencing
title_full_unstemmed Effect of photoperiod on the feline adipose transcriptome as assessed by RNA sequencing
title_short Effect of photoperiod on the feline adipose transcriptome as assessed by RNA sequencing
title_sort effect of photoperiod on the feline adipose transcriptome as assessed by rna sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4092351/
https://www.ncbi.nlm.nih.gov/pubmed/24992939
http://dx.doi.org/10.1186/1746-6148-10-146
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