Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase

5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It is a metabolite of l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. l-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Pan, Zhang, Haiwei, Lv, Min, Hu, Mandong, Li, Zhong, Gao, Chao, Xu, Ping, Ma, Cuiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4093655/
https://www.ncbi.nlm.nih.gov/pubmed/25012259
http://dx.doi.org/10.1038/srep05657
_version_ 1782325766244007936
author Liu, Pan
Zhang, Haiwei
Lv, Min
Hu, Mandong
Li, Zhong
Gao, Chao
Xu, Ping
Ma, Cuiqing
author_facet Liu, Pan
Zhang, Haiwei
Lv, Min
Hu, Mandong
Li, Zhong
Gao, Chao
Xu, Ping
Ma, Cuiqing
author_sort Liu, Pan
collection PubMed
description 5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It is a metabolite of l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. l-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA) play key roles in the biotransformation of l-lysine into 5-aminovalerate. Here, DavB and DavA of P. putida KT2440 were expressed, purified, and coupled for the production of 5-aminovalerate from l-lysine. Under optimal conditions, 20.8 g/L 5-aminovalerate was produced from 30 g/L l-lysine in 12 h. Because l-lysine is an industrial fermentation product, the two-enzyme coupled system presents a promising alternative for the production of 5-aminovalerate.
format Online
Article
Text
id pubmed-4093655
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-40936552014-07-14 Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase Liu, Pan Zhang, Haiwei Lv, Min Hu, Mandong Li, Zhong Gao, Chao Xu, Ping Ma, Cuiqing Sci Rep Article 5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It is a metabolite of l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. l-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA) play key roles in the biotransformation of l-lysine into 5-aminovalerate. Here, DavB and DavA of P. putida KT2440 were expressed, purified, and coupled for the production of 5-aminovalerate from l-lysine. Under optimal conditions, 20.8 g/L 5-aminovalerate was produced from 30 g/L l-lysine in 12 h. Because l-lysine is an industrial fermentation product, the two-enzyme coupled system presents a promising alternative for the production of 5-aminovalerate. Nature Publishing Group 2014-07-11 /pmc/articles/PMC4093655/ /pubmed/25012259 http://dx.doi.org/10.1038/srep05657 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Liu, Pan
Zhang, Haiwei
Lv, Min
Hu, Mandong
Li, Zhong
Gao, Chao
Xu, Ping
Ma, Cuiqing
Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase
title Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase
title_full Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase
title_fullStr Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase
title_full_unstemmed Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase
title_short Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase
title_sort enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4093655/
https://www.ncbi.nlm.nih.gov/pubmed/25012259
http://dx.doi.org/10.1038/srep05657
work_keys_str_mv AT liupan enzymaticproductionof5aminovaleratefromllysineusingllysinemonooxygenaseand5aminovaleramideamidohydrolase
AT zhanghaiwei enzymaticproductionof5aminovaleratefromllysineusingllysinemonooxygenaseand5aminovaleramideamidohydrolase
AT lvmin enzymaticproductionof5aminovaleratefromllysineusingllysinemonooxygenaseand5aminovaleramideamidohydrolase
AT humandong enzymaticproductionof5aminovaleratefromllysineusingllysinemonooxygenaseand5aminovaleramideamidohydrolase
AT lizhong enzymaticproductionof5aminovaleratefromllysineusingllysinemonooxygenaseand5aminovaleramideamidohydrolase
AT gaochao enzymaticproductionof5aminovaleratefromllysineusingllysinemonooxygenaseand5aminovaleramideamidohydrolase
AT xuping enzymaticproductionof5aminovaleratefromllysineusingllysinemonooxygenaseand5aminovaleramideamidohydrolase
AT macuiqing enzymaticproductionof5aminovaleratefromllysineusingllysinemonooxygenaseand5aminovaleramideamidohydrolase

Ejemplares similares