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Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation
This study was conducted to investigate the effects of Trichostatin A (TSA) on cumulus expansion during mouse oocyte maturation. TSA treatment inhibited cumulus expansion and significantly reduced the cumulus expansion index (CEI) (p<0.05). To determine the underlying mechanism, the expression le...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4093811/ https://www.ncbi.nlm.nih.gov/pubmed/25049740 http://dx.doi.org/10.5713/ajas.2013.13128 |
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author | Du, Ming Fu, Xiangwei Zhou, Yanhua Zhu, Shien |
author_facet | Du, Ming Fu, Xiangwei Zhou, Yanhua Zhu, Shien |
author_sort | Du, Ming |
collection | PubMed |
description | This study was conducted to investigate the effects of Trichostatin A (TSA) on cumulus expansion during mouse oocyte maturation. TSA treatment inhibited cumulus expansion and significantly reduced the cumulus expansion index (CEI) (p<0.05). To determine the underlying mechanism, the expression levels of several key factors that play crucial roles in cumulus expansion including components of extracellular matrix (ECM) (Has2, Ptgs2, Ptx3, and Tnfaip6) and Growth differentiation factor 9 (GDF9) were measured in control and TSA treated samples by real-time PCR. The effect of TSA on ERK phosphorylation (p-ERK1/2) in cumulus cells and GDF9 protein level in fully grown oocytes (FGOs) were detected by Western blotting. The expression levels of the ECM genes were significantly decreased (p<0.05) by TSA treatment while GDF9 expression did not response to TSA (p>0.05). TSA treatment blocked the activation of ERK1/2 (p<0.05) and had no significant effect on GDF9 protein expression (p>0.05). Collectively, these results suggested that TSA treatment altered ECM gene expression and blocked ERK1/2 activation to inhibit cumulus expansion in the mouse. |
format | Online Article Text |
id | pubmed-4093811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) |
record_format | MEDLINE/PubMed |
spelling | pubmed-40938112014-07-21 Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation Du, Ming Fu, Xiangwei Zhou, Yanhua Zhu, Shien Asian-Australas J Anim Sci Article This study was conducted to investigate the effects of Trichostatin A (TSA) on cumulus expansion during mouse oocyte maturation. TSA treatment inhibited cumulus expansion and significantly reduced the cumulus expansion index (CEI) (p<0.05). To determine the underlying mechanism, the expression levels of several key factors that play crucial roles in cumulus expansion including components of extracellular matrix (ECM) (Has2, Ptgs2, Ptx3, and Tnfaip6) and Growth differentiation factor 9 (GDF9) were measured in control and TSA treated samples by real-time PCR. The effect of TSA on ERK phosphorylation (p-ERK1/2) in cumulus cells and GDF9 protein level in fully grown oocytes (FGOs) were detected by Western blotting. The expression levels of the ECM genes were significantly decreased (p<0.05) by TSA treatment while GDF9 expression did not response to TSA (p>0.05). TSA treatment blocked the activation of ERK1/2 (p<0.05) and had no significant effect on GDF9 protein expression (p>0.05). Collectively, these results suggested that TSA treatment altered ECM gene expression and blocked ERK1/2 activation to inhibit cumulus expansion in the mouse. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2013-11 /pmc/articles/PMC4093811/ /pubmed/25049740 http://dx.doi.org/10.5713/ajas.2013.13128 Text en Copyright © 2013 by Asian-Australasian Journal of Animal Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/ which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Du, Ming Fu, Xiangwei Zhou, Yanhua Zhu, Shien Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation |
title | Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation |
title_full | Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation |
title_fullStr | Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation |
title_full_unstemmed | Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation |
title_short | Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation |
title_sort | effects of trichostatin a on cumulus expansion during mouse oocyte maturation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4093811/ https://www.ncbi.nlm.nih.gov/pubmed/25049740 http://dx.doi.org/10.5713/ajas.2013.13128 |
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