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Structure Elucidation and Immunomodulatory Activity of A Beta Glucan from the Fruiting Bodies of Ganoderma sinense

A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)– and (1→6)–Glcp, bearing terminal-...

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Autores principales: Han, Xiao-Qiang, Yue, Gar-Lee, Yue, Rui-Qi, Dong, Cai-Xia, Chan, Chung-Lap, Ko, Chun-Hay, Cheung, Wing-Shing, Luo, Ke-Wang, Dai, Hui, Wong, Chun-Kwok, Leung, Ping-Chung, Han, Quan-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094382/
https://www.ncbi.nlm.nih.gov/pubmed/25014571
http://dx.doi.org/10.1371/journal.pone.0100380
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author Han, Xiao-Qiang
Yue, Gar-Lee
Yue, Rui-Qi
Dong, Cai-Xia
Chan, Chung-Lap
Ko, Chun-Hay
Cheung, Wing-Shing
Luo, Ke-Wang
Dai, Hui
Wong, Chun-Kwok
Leung, Ping-Chung
Han, Quan-Bin
author_facet Han, Xiao-Qiang
Yue, Gar-Lee
Yue, Rui-Qi
Dong, Cai-Xia
Chan, Chung-Lap
Ko, Chun-Hay
Cheung, Wing-Shing
Luo, Ke-Wang
Dai, Hui
Wong, Chun-Kwok
Leung, Ping-Chung
Han, Quan-Bin
author_sort Han, Xiao-Qiang
collection PubMed
description A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)– and (1→6)–Glcp, bearing terminal- and (1→3)–Glcp side-chains at O-3 position of (1→6)–Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.
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spelling pubmed-40943822014-07-15 Structure Elucidation and Immunomodulatory Activity of A Beta Glucan from the Fruiting Bodies of Ganoderma sinense Han, Xiao-Qiang Yue, Gar-Lee Yue, Rui-Qi Dong, Cai-Xia Chan, Chung-Lap Ko, Chun-Hay Cheung, Wing-Shing Luo, Ke-Wang Dai, Hui Wong, Chun-Kwok Leung, Ping-Chung Han, Quan-Bin PLoS One Research Article A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)– and (1→6)–Glcp, bearing terminal- and (1→3)–Glcp side-chains at O-3 position of (1→6)–Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells. Public Library of Science 2014-07-11 /pmc/articles/PMC4094382/ /pubmed/25014571 http://dx.doi.org/10.1371/journal.pone.0100380 Text en © 2014 Han et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Han, Xiao-Qiang
Yue, Gar-Lee
Yue, Rui-Qi
Dong, Cai-Xia
Chan, Chung-Lap
Ko, Chun-Hay
Cheung, Wing-Shing
Luo, Ke-Wang
Dai, Hui
Wong, Chun-Kwok
Leung, Ping-Chung
Han, Quan-Bin
Structure Elucidation and Immunomodulatory Activity of A Beta Glucan from the Fruiting Bodies of Ganoderma sinense
title Structure Elucidation and Immunomodulatory Activity of A Beta Glucan from the Fruiting Bodies of Ganoderma sinense
title_full Structure Elucidation and Immunomodulatory Activity of A Beta Glucan from the Fruiting Bodies of Ganoderma sinense
title_fullStr Structure Elucidation and Immunomodulatory Activity of A Beta Glucan from the Fruiting Bodies of Ganoderma sinense
title_full_unstemmed Structure Elucidation and Immunomodulatory Activity of A Beta Glucan from the Fruiting Bodies of Ganoderma sinense
title_short Structure Elucidation and Immunomodulatory Activity of A Beta Glucan from the Fruiting Bodies of Ganoderma sinense
title_sort structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of ganoderma sinense
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094382/
https://www.ncbi.nlm.nih.gov/pubmed/25014571
http://dx.doi.org/10.1371/journal.pone.0100380
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