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Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain
Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequ...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094611/ https://www.ncbi.nlm.nih.gov/pubmed/25071403 |
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author | Gong, Jie Xie, Yinjie Yu, Kun Yang, Ya’nan Huang, Huiyang Ye, Haihui |
author_facet | Gong, Jie Xie, Yinjie Yu, Kun Yang, Ya’nan Huang, Huiyang Ye, Haihui |
author_sort | Gong, Jie |
collection | PubMed |
description | Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5’-promoter region which is 1221 bp in length. Real-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization exhibited the CHY-like reactivity presented in resorptive cells of the hepatopancreas. After bacterial challenge with Vibrio alginolyticus, the expression level of SpCHY mRNA was extremely up-regulated at 3 h in hepatopancreas. Our results suggest that SpCHY might play an important role in the mud crab’s immune response. |
format | Online Article Text |
id | pubmed-4094611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-40946112014-07-28 Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain Gong, Jie Xie, Yinjie Yu, Kun Yang, Ya’nan Huang, Huiyang Ye, Haihui Genet Mol Biol Animal Genetics Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5’-promoter region which is 1221 bp in length. Real-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization exhibited the CHY-like reactivity presented in resorptive cells of the hepatopancreas. After bacterial challenge with Vibrio alginolyticus, the expression level of SpCHY mRNA was extremely up-regulated at 3 h in hepatopancreas. Our results suggest that SpCHY might play an important role in the mud crab’s immune response. Sociedade Brasileira de Genética 2014-06 /pmc/articles/PMC4094611/ /pubmed/25071403 Text en Copyright © 2014, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Animal Genetics Gong, Jie Xie, Yinjie Yu, Kun Yang, Ya’nan Huang, Huiyang Ye, Haihui Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain |
title | Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain |
title_full | Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain |
title_fullStr | Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain |
title_full_unstemmed | Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain |
title_short | Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain |
title_sort | characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, scylla paramamosain |
topic | Animal Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094611/ https://www.ncbi.nlm.nih.gov/pubmed/25071403 |
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