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Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed
Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the “river buffaloes” with 2n = 50 and the “swamp buffaloes”, 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these dif...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094616/ https://www.ncbi.nlm.nih.gov/pubmed/25071402 |
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author | Degrandi, Tiago Marafiga Pita, Sebastian Panzera, Yanina de Oliveira, Edivaldo Herculano C. Marques, José Ribamar Felipe Figueiró, Marivaldo Rodrigues Marques, Larissa Coêlho Vinadé, Lucia Gunski, Ricardo José Garnero, Analía Del Valle |
author_facet | Degrandi, Tiago Marafiga Pita, Sebastian Panzera, Yanina de Oliveira, Edivaldo Herculano C. Marques, José Ribamar Felipe Figueiró, Marivaldo Rodrigues Marques, Larissa Coêlho Vinadé, Lucia Gunski, Ricardo José Garnero, Analía Del Valle |
author_sort | Degrandi, Tiago Marafiga |
collection | PubMed |
description | Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the “river buffaloes” with 2n = 50 and the “swamp buffaloes”, 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 cross-breed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies. |
format | Online Article Text |
id | pubmed-4094616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-40946162014-07-28 Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed Degrandi, Tiago Marafiga Pita, Sebastian Panzera, Yanina de Oliveira, Edivaldo Herculano C. Marques, José Ribamar Felipe Figueiró, Marivaldo Rodrigues Marques, Larissa Coêlho Vinadé, Lucia Gunski, Ricardo José Garnero, Analía Del Valle Genet Mol Biol Animal Genetics Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the “river buffaloes” with 2n = 50 and the “swamp buffaloes”, 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 cross-breed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies. Sociedade Brasileira de Genética 2014-06 /pmc/articles/PMC4094616/ /pubmed/25071402 Text en Copyright © 2014, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Animal Genetics Degrandi, Tiago Marafiga Pita, Sebastian Panzera, Yanina de Oliveira, Edivaldo Herculano C. Marques, José Ribamar Felipe Figueiró, Marivaldo Rodrigues Marques, Larissa Coêlho Vinadé, Lucia Gunski, Ricardo José Garnero, Analía Del Valle Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed |
title | Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed |
title_full | Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed |
title_fullStr | Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed |
title_full_unstemmed | Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed |
title_short | Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed |
title_sort | karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed |
topic | Animal Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094616/ https://www.ncbi.nlm.nih.gov/pubmed/25071402 |
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