Cargando…

Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants

This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically ana...

Descripción completa

Detalles Bibliográficos
Autores principales: Granada, Camille E., Strochein, Marcos, Vargas, Luciano K., Bruxel, Manuela, de Sá, Enilson Luiz Saccol, Passaglia, Luciane M.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094621/
https://www.ncbi.nlm.nih.gov/pubmed/25071405
_version_ 1782325867620335616
author Granada, Camille E.
Strochein, Marcos
Vargas, Luciano K.
Bruxel, Manuela
de Sá, Enilson Luiz Saccol
Passaglia, Luciane M.P.
author_facet Granada, Camille E.
Strochein, Marcos
Vargas, Luciano K.
Bruxel, Manuela
de Sá, Enilson Luiz Saccol
Passaglia, Luciane M.P.
author_sort Granada, Camille E.
collection PubMed
description This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.
format Online
Article
Text
id pubmed-4094621
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Sociedade Brasileira de Genética
record_format MEDLINE/PubMed
spelling pubmed-40946212014-07-28 Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants Granada, Camille E. Strochein, Marcos Vargas, Luciano K. Bruxel, Manuela de Sá, Enilson Luiz Saccol Passaglia, Luciane M.P. Genet Mol Biol Plant Genetics This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species. Sociedade Brasileira de Genética 2014-06 /pmc/articles/PMC4094621/ /pubmed/25071405 Text en Copyright © 2014, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Plant Genetics
Granada, Camille E.
Strochein, Marcos
Vargas, Luciano K.
Bruxel, Manuela
de Sá, Enilson Luiz Saccol
Passaglia, Luciane M.P.
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_full Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_fullStr Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_full_unstemmed Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_short Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_sort genetic diversity and symbiotic compatibility among rhizobial strains and desmodium incanum and lotus spp. plants
topic Plant Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094621/
https://www.ncbi.nlm.nih.gov/pubmed/25071405
work_keys_str_mv AT granadacamillee geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants
AT strocheinmarcos geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants
AT vargaslucianok geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants
AT bruxelmanuela geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants
AT desaenilsonluizsaccol geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants
AT passaglialucianemp geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants