Cargando…
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically ana...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094621/ https://www.ncbi.nlm.nih.gov/pubmed/25071405 |
_version_ | 1782325867620335616 |
---|---|
author | Granada, Camille E. Strochein, Marcos Vargas, Luciano K. Bruxel, Manuela de Sá, Enilson Luiz Saccol Passaglia, Luciane M.P. |
author_facet | Granada, Camille E. Strochein, Marcos Vargas, Luciano K. Bruxel, Manuela de Sá, Enilson Luiz Saccol Passaglia, Luciane M.P. |
author_sort | Granada, Camille E. |
collection | PubMed |
description | This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species. |
format | Online Article Text |
id | pubmed-4094621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-40946212014-07-28 Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants Granada, Camille E. Strochein, Marcos Vargas, Luciano K. Bruxel, Manuela de Sá, Enilson Luiz Saccol Passaglia, Luciane M.P. Genet Mol Biol Plant Genetics This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species. Sociedade Brasileira de Genética 2014-06 /pmc/articles/PMC4094621/ /pubmed/25071405 Text en Copyright © 2014, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Plant Genetics Granada, Camille E. Strochein, Marcos Vargas, Luciano K. Bruxel, Manuela de Sá, Enilson Luiz Saccol Passaglia, Luciane M.P. Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title | Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_full | Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_fullStr | Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_full_unstemmed | Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_short | Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_sort | genetic diversity and symbiotic compatibility among rhizobial strains and desmodium incanum and lotus spp. plants |
topic | Plant Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094621/ https://www.ncbi.nlm.nih.gov/pubmed/25071405 |
work_keys_str_mv | AT granadacamillee geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants AT strocheinmarcos geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants AT vargaslucianok geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants AT bruxelmanuela geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants AT desaenilsonluizsaccol geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants AT passaglialucianemp geneticdiversityandsymbioticcompatibilityamongrhizobialstrainsanddesmodiumincanumandlotussppplants |