Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1

Early diagnosis and prognosis monitoring are very important for the survival of patients with bladder cancer. To identify candidate biomarkers of bladder cancer, we used a combination of techniques including 2-DE, co-IP, western blot, LC-MS/MS, and immunohistochemistry. Hsp74 was identified with hig...

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Detalles Bibliográficos
Autores principales: Chen, Ling, Wang, YaRong, Zhao, Le, Chen, Wei, Dong, Chunhui, Zhao, Xinhan, Li, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094855/
https://www.ncbi.nlm.nih.gov/pubmed/25050384
http://dx.doi.org/10.1155/2014/492849
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author Chen, Ling
Wang, YaRong
Zhao, Le
Chen, Wei
Dong, Chunhui
Zhao, Xinhan
Li, Xu
author_facet Chen, Ling
Wang, YaRong
Zhao, Le
Chen, Wei
Dong, Chunhui
Zhao, Xinhan
Li, Xu
author_sort Chen, Ling
collection PubMed
description Early diagnosis and prognosis monitoring are very important for the survival of patients with bladder cancer. To identify candidate biomarkers of bladder cancer, we used a combination of techniques including 2-DE, co-IP, western blot, LC-MS/MS, and immunohistochemistry. Hsp74 was identified with high expression in bladder cancer. The cellular location of expression products of gene Hsp74 showed that they were distributed into cytoplasm and keratin 1 was found to be associated with Hsp74. The results provide a new idea to understand the molecular basis of bladder cancer progression and pinpoint new potential molecular target for early diagnosis and therapeutic monitoring of bladder cancer.
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spelling pubmed-40948552014-07-21 Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1 Chen, Ling Wang, YaRong Zhao, Le Chen, Wei Dong, Chunhui Zhao, Xinhan Li, Xu J Immunol Res Research Article Early diagnosis and prognosis monitoring are very important for the survival of patients with bladder cancer. To identify candidate biomarkers of bladder cancer, we used a combination of techniques including 2-DE, co-IP, western blot, LC-MS/MS, and immunohistochemistry. Hsp74 was identified with high expression in bladder cancer. The cellular location of expression products of gene Hsp74 showed that they were distributed into cytoplasm and keratin 1 was found to be associated with Hsp74. The results provide a new idea to understand the molecular basis of bladder cancer progression and pinpoint new potential molecular target for early diagnosis and therapeutic monitoring of bladder cancer. Hindawi Publishing Corporation 2014 2014-06-23 /pmc/articles/PMC4094855/ /pubmed/25050384 http://dx.doi.org/10.1155/2014/492849 Text en Copyright © 2014 Ling Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Ling
Wang, YaRong
Zhao, Le
Chen, Wei
Dong, Chunhui
Zhao, Xinhan
Li, Xu
Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1
title Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1
title_full Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1
title_fullStr Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1
title_full_unstemmed Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1
title_short Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1
title_sort hsp74, a potential bladder cancer marker, has direct interaction with keratin 1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094855/
https://www.ncbi.nlm.nih.gov/pubmed/25050384
http://dx.doi.org/10.1155/2014/492849
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