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Modulation of P-selection and platelet aggregation in chronic periodontitis: A clinical study

BACKGROUND: The primary etiologic factor of periodontitis is the subgingival infection with a group of Gram negative pathogens. Transient bacteremia in periodontitis patients underlie chronic production and systemic increases of various proinflammatory mediators, including Interleukin (IL)-1α, IL-6,...

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Autores principales: Perumal, Ramesh, Rajendran, Maheashwari, Krishnamurthy, Malathi, Ganji, Kiran Kumar, Pendor, Sunil Dattuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4095619/
https://www.ncbi.nlm.nih.gov/pubmed/25024540
http://dx.doi.org/10.4103/0972-124X.134563
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author Perumal, Ramesh
Rajendran, Maheashwari
Krishnamurthy, Malathi
Ganji, Kiran Kumar
Pendor, Sunil Dattuji
author_facet Perumal, Ramesh
Rajendran, Maheashwari
Krishnamurthy, Malathi
Ganji, Kiran Kumar
Pendor, Sunil Dattuji
author_sort Perumal, Ramesh
collection PubMed
description BACKGROUND: The primary etiologic factor of periodontitis is the subgingival infection with a group of Gram negative pathogens. Transient bacteremia in periodontitis patients underlie chronic production and systemic increases of various proinflammatory mediators, including Interleukin (IL)-1α, IL-6, C-reactive protein and Tumor necrosis factor (TNF)-α. P- selectin is a member of selectin family of cell surface receptor which is located in the membrane of the secretory granules (alpha granules) of platelets and in the membrane of the Weibel-Palade bodies of the vascular endothelial cells. P selectin redistributes from the membrane of the granules to the plasma membrane when platelets and endothelial cells are activated and thus degranulated. AIM: To compare the level of platelet activation, soluble P Selectin level and morphological changes and aggregation of platelets in patients in periodontitis patients compared to healthy controls. MATERIALS AND METHODS: 80 patients were included in the study with the age group of 35-60. The patients were divided into 2 groups, 40 subjects with generalized chronic periodontitis and 40 healthy subjects taken as control. Periodontal Examination using clinical parameters namely, Bleeding Index, Plaque Index, Probing Pocket Depth and Clinical Attachment Level were recorded. Collection of blood samples for estimation of serum soluble P- selectin level by ELISA method. Evaluation of Platelet morphology and grading the platelet aggregation. RESULTS: P-selectin expression shows that the mean value for control group is 4.97 ± 16.56 ng/mL and study group 13.05 ± 29.94 ng/mL which was significantly higher than control group with P value 0.001. Platelet morphological changes shows small form – mean value for control group is 75.83% ± 14.24% while for study group is 39.08%. ± 21.59; Big form – mean value for control group 0.80% ± 0.35% while for study group 0.48% ± 1.3%and Spider form- mean value for control group 23.88% ± 14.13 while study group 59.32% ±. 23.42. The observation showed high statistical significance with P- value < 0.001 for small and spider form and no statistical significance for big form P = 0.075. CONCLUSION: Increased expression of P-selectin, spider form of platelets and pathological aggregation pattern which indicates that platelet activation may be associated with chronic periodontitis. The results of the study showed, higher number of spider forms and significant pathological aggregation pattern in periodontitis patients which indicates activation of platelets thus emphasized that periodontitis can be an contributing factor in the development of cardiovascular disease.
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spelling pubmed-40956192014-07-14 Modulation of P-selection and platelet aggregation in chronic periodontitis: A clinical study Perumal, Ramesh Rajendran, Maheashwari Krishnamurthy, Malathi Ganji, Kiran Kumar Pendor, Sunil Dattuji J Indian Soc Periodontol Original Article BACKGROUND: The primary etiologic factor of periodontitis is the subgingival infection with a group of Gram negative pathogens. Transient bacteremia in periodontitis patients underlie chronic production and systemic increases of various proinflammatory mediators, including Interleukin (IL)-1α, IL-6, C-reactive protein and Tumor necrosis factor (TNF)-α. P- selectin is a member of selectin family of cell surface receptor which is located in the membrane of the secretory granules (alpha granules) of platelets and in the membrane of the Weibel-Palade bodies of the vascular endothelial cells. P selectin redistributes from the membrane of the granules to the plasma membrane when platelets and endothelial cells are activated and thus degranulated. AIM: To compare the level of platelet activation, soluble P Selectin level and morphological changes and aggregation of platelets in patients in periodontitis patients compared to healthy controls. MATERIALS AND METHODS: 80 patients were included in the study with the age group of 35-60. The patients were divided into 2 groups, 40 subjects with generalized chronic periodontitis and 40 healthy subjects taken as control. Periodontal Examination using clinical parameters namely, Bleeding Index, Plaque Index, Probing Pocket Depth and Clinical Attachment Level were recorded. Collection of blood samples for estimation of serum soluble P- selectin level by ELISA method. Evaluation of Platelet morphology and grading the platelet aggregation. RESULTS: P-selectin expression shows that the mean value for control group is 4.97 ± 16.56 ng/mL and study group 13.05 ± 29.94 ng/mL which was significantly higher than control group with P value 0.001. Platelet morphological changes shows small form – mean value for control group is 75.83% ± 14.24% while for study group is 39.08%. ± 21.59; Big form – mean value for control group 0.80% ± 0.35% while for study group 0.48% ± 1.3%and Spider form- mean value for control group 23.88% ± 14.13 while study group 59.32% ±. 23.42. The observation showed high statistical significance with P- value < 0.001 for small and spider form and no statistical significance for big form P = 0.075. CONCLUSION: Increased expression of P-selectin, spider form of platelets and pathological aggregation pattern which indicates that platelet activation may be associated with chronic periodontitis. The results of the study showed, higher number of spider forms and significant pathological aggregation pattern in periodontitis patients which indicates activation of platelets thus emphasized that periodontitis can be an contributing factor in the development of cardiovascular disease. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4095619/ /pubmed/25024540 http://dx.doi.org/10.4103/0972-124X.134563 Text en Copyright: © Journal of Indian Society of Periodontology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Perumal, Ramesh
Rajendran, Maheashwari
Krishnamurthy, Malathi
Ganji, Kiran Kumar
Pendor, Sunil Dattuji
Modulation of P-selection and platelet aggregation in chronic periodontitis: A clinical study
title Modulation of P-selection and platelet aggregation in chronic periodontitis: A clinical study
title_full Modulation of P-selection and platelet aggregation in chronic periodontitis: A clinical study
title_fullStr Modulation of P-selection and platelet aggregation in chronic periodontitis: A clinical study
title_full_unstemmed Modulation of P-selection and platelet aggregation in chronic periodontitis: A clinical study
title_short Modulation of P-selection and platelet aggregation in chronic periodontitis: A clinical study
title_sort modulation of p-selection and platelet aggregation in chronic periodontitis: a clinical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4095619/
https://www.ncbi.nlm.nih.gov/pubmed/25024540
http://dx.doi.org/10.4103/0972-124X.134563
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