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The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions

A major part of membrane function is conducted by proteins, both integral and peripheral. Peripheral membrane proteins temporarily adhere to biological membranes, either to the lipid bilayer or to integral membrane proteins with noncovalent interactions. The aim of this study was to construct and an...

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Detalles Bibliográficos
Autores principales: Nastou, Katerina C., Tsaousis, Georgios N., Kremizas, Kimon E., Litou, Zoi I., Hamodrakas, Stavros J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4095733/
https://www.ncbi.nlm.nih.gov/pubmed/25057483
http://dx.doi.org/10.1155/2014/397145
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author Nastou, Katerina C.
Tsaousis, Georgios N.
Kremizas, Kimon E.
Litou, Zoi I.
Hamodrakas, Stavros J.
author_facet Nastou, Katerina C.
Tsaousis, Georgios N.
Kremizas, Kimon E.
Litou, Zoi I.
Hamodrakas, Stavros J.
author_sort Nastou, Katerina C.
collection PubMed
description A major part of membrane function is conducted by proteins, both integral and peripheral. Peripheral membrane proteins temporarily adhere to biological membranes, either to the lipid bilayer or to integral membrane proteins with noncovalent interactions. The aim of this study was to construct and analyze the interactions of the human plasma membrane peripheral proteins (peripherome hereinafter). For this purpose, we collected a dataset of peripheral proteins of the human plasma membrane. We also collected a dataset of experimentally verified interactions for these proteins. The interaction network created from this dataset has been visualized using Cytoscape. We grouped the proteins based on their subcellular location and clustered them using the MCL algorithm in order to detect functional modules. Moreover, functional and graph theory based analyses have been performed to assess biological features of the network. Interaction data with drug molecules show that ~10% of peripheral membrane proteins are targets for approved drugs, suggesting their potential implications in disease. In conclusion, we reveal novel features and properties regarding the protein-protein interaction network created by peripheral proteins of the human plasma membrane.
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spelling pubmed-40957332014-07-23 The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions Nastou, Katerina C. Tsaousis, Georgios N. Kremizas, Kimon E. Litou, Zoi I. Hamodrakas, Stavros J. Biomed Res Int Research Article A major part of membrane function is conducted by proteins, both integral and peripheral. Peripheral membrane proteins temporarily adhere to biological membranes, either to the lipid bilayer or to integral membrane proteins with noncovalent interactions. The aim of this study was to construct and analyze the interactions of the human plasma membrane peripheral proteins (peripherome hereinafter). For this purpose, we collected a dataset of peripheral proteins of the human plasma membrane. We also collected a dataset of experimentally verified interactions for these proteins. The interaction network created from this dataset has been visualized using Cytoscape. We grouped the proteins based on their subcellular location and clustered them using the MCL algorithm in order to detect functional modules. Moreover, functional and graph theory based analyses have been performed to assess biological features of the network. Interaction data with drug molecules show that ~10% of peripheral membrane proteins are targets for approved drugs, suggesting their potential implications in disease. In conclusion, we reveal novel features and properties regarding the protein-protein interaction network created by peripheral proteins of the human plasma membrane. Hindawi Publishing Corporation 2014 2014-06-25 /pmc/articles/PMC4095733/ /pubmed/25057483 http://dx.doi.org/10.1155/2014/397145 Text en Copyright © 2014 Katerina C. Nastou et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nastou, Katerina C.
Tsaousis, Georgios N.
Kremizas, Kimon E.
Litou, Zoi I.
Hamodrakas, Stavros J.
The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions
title The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions
title_full The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions
title_fullStr The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions
title_full_unstemmed The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions
title_short The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions
title_sort human plasma membrane peripherome: visualization and analysis of interactions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4095733/
https://www.ncbi.nlm.nih.gov/pubmed/25057483
http://dx.doi.org/10.1155/2014/397145
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