SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained ch...

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Detalles Bibliográficos
Autores principales: Upadhyay, Santosh Kumar, Sharma, Shailesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4095993/
https://www.ncbi.nlm.nih.gov/pubmed/25089276
http://dx.doi.org/10.1155/2014/742482
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author Upadhyay, Santosh Kumar
Sharma, Shailesh
author_facet Upadhyay, Santosh Kumar
Sharma, Shailesh
author_sort Upadhyay, Santosh Kumar
collection PubMed
description Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained challenging. Here, we report SSFinder, a python script to perform high throughput detection of specific target sites in large nucleotide datasets. The SSFinder is a user-friendly tool, compatible with Windows, Mac OS, and Linux operating systems, and freely available online.
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spelling pubmed-40959932014-08-03 SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool Upadhyay, Santosh Kumar Sharma, Shailesh Biomed Res Int Research Article Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained challenging. Here, we report SSFinder, a python script to perform high throughput detection of specific target sites in large nucleotide datasets. The SSFinder is a user-friendly tool, compatible with Windows, Mac OS, and Linux operating systems, and freely available online. Hindawi Publishing Corporation 2014 2014-06-26 /pmc/articles/PMC4095993/ /pubmed/25089276 http://dx.doi.org/10.1155/2014/742482 Text en Copyright © 2014 S. K. Upadhyay and S. Sharma. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Upadhyay, Santosh Kumar
Sharma, Shailesh
SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool
title SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool
title_full SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool
title_fullStr SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool
title_full_unstemmed SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool
title_short SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool
title_sort ssfinder: high throughput crispr-cas target sites prediction tool
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4095993/
https://www.ncbi.nlm.nih.gov/pubmed/25089276
http://dx.doi.org/10.1155/2014/742482
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AT sharmashailesh ssfinderhighthroughputcrisprcastargetsitespredictiontool

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